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Caffeine as a semen additive to improve Murrah buffalo (Bubalus bubalis) semen cryopreservation |
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| รหัสดีโอไอ | |
| Creator | 1. Shukla, M.K. 2. Misra, A.K. |
| Title | Caffeine as a semen additive to improve Murrah buffalo (Bubalus bubalis) semen cryopreservation |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2557 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 33 |
| Journal No. | 1 |
| Page no. | 32-36 |
| Keyword | Murrah buffaloes,Bubalus bubalis,Semen,Stimulant,Caffeine,Cryopreservation,Egg yolk,EYTG,India |
| ISSN | 1256726 |
| Abstract | Caffeine was incorporated in egg yolk tris glycerol extender (EYTG) at the time of dilution of Murrah semen at a concentration of 1.0, 3.0 or 5.0 mM. The samples were subsequently frozen. The post thaw progressive motility and live sperm per cent improved signifi cantly (P LT 0.05) with addition of 1.0 (70.0+-0.99 and 88.80+-0.84) and 3.0 mΜ(65.83+-0.88 and 86.16+-0.96 percent) caffeine to the extender before semen dilution and cryopreservation as compared to the control (61.94+-1.15 and 79.05+-1.02 percent), when evaluated at 0 h post freeze. All the three concentrations of caffeine signifi cantly improved the hypoosmotic swelling (HOS) percent and protected the sperm from becoming abnormal as observed at 0 and 48 h after freezing. Thus incorporation of caffeine (1.0 or 3.0 mM) in EYTG extender, prior to freezing may be useful in improving the quality of cryopreserved bubaline semen. |
