Distribution of Salmonella species in buffaloes in some middle governorates of Iraq
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Creator 1. Afaf Abdulrahman Yousif
2. Ali, Al-Hashimi D.M.
Title Distribution of Salmonella species in buffaloes in some middle governorates of Iraq
Publisher International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand)
Publication Year 2557
Journal Title Buffalo Bulletin
Journal Vol. 33
Journal No. 3
Page no. 337-349
Keyword Buffaloes,Bubalus bubalis,Salmonella,Infection,Clinical signs,Iraq
ISSN 1256726
Abstract This study was planned to isolate and type Salmonella from buffalo in three middle governorates in Iraq. One hundred fifty milk samples were collected from 150 lactating buffaloes in the field, and in the slaughter house (150) fecal samples were collected at slaughter time and (900) sample were collected post slaughtering:150 samples from each organ: gall bladder, mesenteric lymph nodes, spleen, liver, small intestine and cecum Diagnostic study depended upon the morphological and cultural properties of the isolates on some selective media like Brilliant Green , XLD, SS agar and Hicrome rajhans medium, modified (Salmonella agar modified) were used in addition to different biochemical tests, API-20E and KB 003 Hi25 TM Enterobacteriaceae Identification Kit, latex test and serotyping of isolates. Twenty-two isolates were obtained from the fecal samples and organs, these isolates belonged to three serotypes (Salmonella anatum (68.18 percent), S.muenchen (18.18 percent) and S. enteritidis (13.64 percent), while no isolate was obtained from milk samples. In animals from slaughter houses, the percentage of infection varied in examined organs and feces: in bile duct and liver, the percentage was 3.33 percent each, in mesenteric lymph nodes, 2.67 percent, in spleen and cecum, 2 percent each, and in feces, 1.33 percent. The antibiotic susceptibility pattern of Salmonella against 15 antimicrobial drugs revealed that all isolates were resistant (100 percent) to chloramphenicol, suphamethoxazole, erythromycin, cloxacillin, and tetracycline, and all isolates were sensitive to amicacin and trimethoprimwhile neomycine, gentamycine, cefixime, ciprofloxacin, kanamycin and streptomycin gave intermediate results.
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