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Echinococcus granulosus antigen B for serodiagnosois of cystic echinococcosis in buffalo (Bubalus bubalis) |
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| รหัสดีโอไอ | |
| Creator | 1. Maity, A. 2. Bhattacharya, D. 3. Batabyal, S. 4. Chattopadhyay, S. 5. Bera, A.K. |
| Title | Echinococcus granulosus antigen B for serodiagnosois of cystic echinococcosis in buffalo (Bubalus bubalis) |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2557 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 33 |
| Journal No. | 1 |
| Page no. | 94-100 |
| Keyword | Buffaloes,Bubalus bubalis,Cystic echinococcosis,Antigen B,Echinococcus granulosus,Serodiagnosis,India |
| ISSN | 1256726 |
| Abstract | The present investigation was carried out to characterize buffalo cyst fluid and germinal membrane antigen by a double immuno diffusion test and immunoelectrophoresis. Attempts were also made to fi nd out utility of antigen B for detection of cystic echinococcosis in buffalo by indirect enzyme linked immunosorbent assay (indirect ELISA). Hyperimmune sera raised against germinal membrane antigen and cyst fluid antigen has shown seroreactivity during the double immuno diffusion test and immunoelectrophoresis against germinal membrane antigen, cyst fluid antigen respectively as apparent by formation of precipitin lines and precipitation arc. A total of twenty sera samples (ten positive and negative samples each) of buffalo were used for indirect ELISA and the average optical density (O.D.) values of positive samples ranged from 0.498 to 0.783 with a mean of 0.655. The negative samples exhibited O.D. values ranged from 0.247 to 0.263 with a mean of 0.255. The cut-off value of the test system was 0.279. |
