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Effects of addition of taurine to semen extenders on microscopic factors of buffalo bulls sperm after thawing |
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| รหัสดีโอไอ | |
| Creator | 1. Beheshti, R. 2. Yosefi-Asl, M. 3. Eshratkhah, B. 4. Ghiasi Ghale-Kandi, J. |
| Title | Effects of addition of taurine to semen extenders on microscopic factors of buffalo bulls sperm after thawing |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2556 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 32 |
| Journal No. | 1 |
| Page no. | 46-52 |
| Keyword | Buffalo bulls,Bubalus bubalis,Semen,Extender,Taurine,Microscopic parameters,Antioxidant,Iran |
| ISSN | 1256726 |
| Abstract | The aim of this study was to evaluate the effects of the addition of different levels of antioxidant (taurine) on buffalo spermatozoa cryopreserved in an egg yolk based extender. Ejaculates were collected from fi ve mature buffalo bulls (four ejaculates per buffalo) and were evaluated and diluted at 37 deg C in one of the following experiments: tris-egg yolk extender (control), or the same extender supplemented with either 10, 25, 50, 75 or 100 mM taurine. The semen was loaded into 0.5 ml straws, cooled and frozen in a programmable freezer and subsequently stored in liquid nitrogen. Prior to evaluation, frozen straws were thawed in a water bath (37 deg C for 20 s). Freezing extenders supplemented with 25 and 10,100 mM taurine led to higher and lower sperm motility values (respectively), compared to the control (P LT 0.05) and following the freeze-thawing process. The addition of antioxidants did not affect acrosomal integrity compared to the control. |
