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Evaluation of follicular atresia and electrophoretic pattern of follicular fluid proteins in acyclic buffalo (Bubalus bubalis) |
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| รหัสดีโอไอ | |
| Creator | 1. Khan, F.A. 2. Das, G.K. 3. Megha Pande 4. Rajendra Singh 5. Ghosh, S.K. |
| Title | Evaluation of follicular atresia and electrophoretic pattern of follicular fluid proteins in acyclic buffalo (Bubalus bubalis) |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2556 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 32 |
| Journal No. | 4 |
| Page no. | 265-269 |
| Keyword | Buffaloes,Bubalus bubalis,Ovary follicle,Atresia,Follicular fluid,USA |
| ISSN | 1256726 |
| Abstract | Histological examination of H and E stained sections of ovaries collected from cyclic and acyclicbuffaloes (n=6/group) was done in order to evaluate the degree of follicular atresia. The percentages of healthy and atretic follicles were different (P LT 0.05) between cyclic (53.1 percent and 46.9 percent, respectively) and acyclic (10.7 percent and 89.3 percent, respectively) buffaloes. Electrophoretic patterns of follicular fl uid proteins, studied by SDS-PAGE of follicular fluid samples aspirated from small (5.0-6.9 mm), medium (7.0-9.9 mm) and large (GT= 10 mm) follicles of cyclic and acyclic buffaloes (n=6/group), did not reveal any apparent differences between the groups. DNA fragmentation patterns evaluated by using DNA isolated from the cell pellets obtained after centrifugation of the follicular fluid samples from small-, medium- and largesized follicles of cyclic and acyclic buffaloes (n=5/group) showed a typical apoptotic oligonucleosome ladder pattern in the acyclic group. In conclusion, these results indicate an increased rate of follicular atresia without any qualitative changes in the follicular fluid proteins during ovarian acyclicity in buffalo. |
