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Oxidized low density lipoprotein receptor 1 (OLR 1) gene polymorphism in Bubalus bubalis |
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| รหัสดีโอไอ | |
| Creator | 1. Manisha Deshpande 2. Rank, D.N. 3. Vataliya, P.H. 4. Joshi, C.G. |
| Title | Oxidized low density lipoprotein receptor 1 (OLR 1) gene polymorphism in Bubalus bubalis |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2556 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 32 |
| Journal No. | 4 |
| Page no. | 260-264 |
| Keyword | Mehsana buffaloes,Bubalus bubalis PCRRFLP,OLR1 gene,Polymorphism,India |
| ISSN | 1256726 |
| Abstract | Oxidized low density lipoprotein receptor 1 (OLR1) is the major protein that binds, internalizes and degrades oxidized low-density lipoprotein. It is reported that SNP 8,232 in the 3'-UTR in OLR1 was associated with milk fat yield and percentage. This study was aimed to reveal the PCR-RFLP pattern of OLR1 (3'UTR) in the Mehsana breed of buffaloes. A fragment of OLR1-288 bp was amplifi ed by PCR, and subsequently, RFLP study was carried out to identify genotypes of the animals with PstI restriction enzyme. It revealed monomorphic patterns. Further representative samples were cloned and in vector and after sequencing sequence variation in nucleotide sequences of OLR1was analysed. On comparison with published cattle sequence the nucleotide sequence variation between cattle and buffalo was present at nine nucleotide positions i.e. 85, 91, 116, 129, 151, 168, 171, 217, 240. |
