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Molecular characterization of the leptin gene in riverine buffaloes |
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| รหัสดีโอไอ | |
| Creator | 1. Sanjoy Datta 2. Archana Verma 3. Paresh Chatterjee 4. Aruna Pal Chatterjee |
| Title | Molecular characterization of the leptin gene in riverine buffaloes |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2556 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 32 |
| Journal No. | 3 |
| Page no. | 196-211 |
| Keyword | Buffaloes,Bubalus bubalis,Leptin gene,PCR-RFLP,Genetic polymorphism,Phylogenetic tree,Haplotype,India |
| ISSN | 1256726 |
| Abstract | The present study was undertaken with the objectives of sequence characterization and studying the genetic variation in leptin gene locus 2nd exon and 3rd exon in Murrah buffalo by PCRRFLP. The leptin gene was amplified by PCR using oligonucleotide primers standardized for Bos taurus species. 289 bp fragment comprising of exon 2 and 405 bp fragment containing exon 3 of leptin gene were amplified and digested with Alu I , AciI, MspI, Sau3AI, HphI restriction enzyme using a cattle specific primer. The sizes of the amplification products were similar in cattle and buffalo. The leptin gene in buffalo reveals monomorphism since no variation was found. The result indicates strong conservation of DNA sequence between cattle and buffalo. Nucleotide sequence variations observed in the leptin gene between Bubalus bubalis and Bos taurus species revealed 97.0 percent nucleotide identity. Sequence comparison of buffalo with cattle reveals variation at eight nucleotide sequences at positions 983, 1083, 1147, 1152, 1221, 1371, 3318, 3333 when all the SNPs are synonymous resulting in no change in amino acids. The milk production traits namely milk yield and fat yield could not be associated with buffalo leptin genotypes due to their monomorphic haplotype. |
