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Genetic Variation and Polymorphism Identification of the Thyroglobulin (TG) Gene in Thai Wagyu Crossbred Cattle |
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| รหัสดีโอไอ | |
| Creator | Chanachotintawit Kaopong |
| Title | Genetic Variation and Polymorphism Identification of the Thyroglobulin (TG) Gene in Thai Wagyu Crossbred Cattle |
| Contributor | Jiratti Thammasiri, Anupong Tankrathok, Jiranan Insee |
| Publisher | สถาบันวิจัยและพัฒนา มหาวิทยาลัยกาฬสินธุ์ |
| Publication Year | 2568 |
| Journal Title | วารสารวิทยาศาสตร์ เทคโนโลยี และนวัตกรรม มหาวิทยาลัยกาฬสินธุ์ |
| Journal Vol. | 4 |
| Journal No. | 2 |
| Page no. | 165-174 |
| Keyword | Genetic variation, Thyroglobulin (TG) gene, Wagyu Crossbred Cattle |
| URL Website | https://li01.tci-thaijo.org/index.php/sci_01/article/view/269654 |
| Website title | วารสารวิทยาศาสตร์ เทคโนโลยี และนวัตกรรม มหาวิทยาลัยกาฬสินธุ์ |
| ISSN | 2821-9406 (Online) |
| Abstract | The thyroglobulin (TG) gene is recognized as a major candidate gene involved in regulating intramuscular fat (IMF) deposition, a key determinant of beef quality. This study aimed to evaluate the genetic diversity and identify novel polymorphisms of the TG gene in Thai Wagyu crossbred cattle. Blood samples (n = 50) were collected from male animals to extract genomic DNA (gDNA). The target TG gene fragment was amplified using the polymerase chain reaction (PCR) and subsequently sequenced to identify single nucleotide polymorphisms (SNPs) and estimate population genetic parameters. Seven SNPs were identified including g.164A>G, g.257C>T, g.335A>G, g.386C>T, g.422C>T, g.537C>T, and g.552T>C. The expected heterozygosity (He) ranged from 0.41 to 0.49, while the polymorphic information content (PIC) ranged from 0.32 to 0.37, indicating moderate genetic diversity. The population was in Hardy–Weinberg equilibrium (HWE) (P > 0.05). The discovery of these seven novel SNPs provides essential baseline data for future association studies with meat quality traits and supports the potential application of TG gene polymorphisms as molecular markers in marker-assisted selection (MAS) programs aimed at improving meat quality in Wagyu crossbred cattle. |
