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การผลิตเอทานอลที่มีความบริสุทธิ์สูงจากหญ้าเนเปียร์ที่มีการปรับสภาพ |
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| รหัสดีโอไอ | |
| Creator | ภัทรมาศ เทียมเงิน |
| Title | การผลิตเอทานอลที่มีความบริสุทธิ์สูงจากหญ้าเนเปียร์ที่มีการปรับสภาพ |
| Contributor | ธีระวัฒน์ เหมือนศรีชัย, ชัยภพ ศิระวรกุล, ชนิกานต์ ทองปาน, น้ำทิพย์ วันดี, พีรวัส สุยะทา |
| Publisher | King Mongkut’s University of Technology Thonburi |
| Publication Year | 2566 |
| Journal Title | KMUTT Research and Development Journal |
| Journal Vol. | 46 |
| Journal No. | 1 |
| Page no. | 55-66 |
| Keyword | Ethanol, Napier Grass, Saccharomyces Cerevisiae, Crude Enzyme, Molecular Sieve |
| URL Website | https://journal.kmutt.ac.th/ |
| Website title | เว็บไซต์วารสารวิจัยและพัฒนา มจธ. |
| ISSN | 2697-5521 |
| Abstract | Napier grass Pakchong 1 (Pennisetum purpureum x Pennisetum americanum) was used as a raw material for the production of ethanol. Trichoderma reesei TISTR3080 and cellulase enzyme were used for the hydrolysis, while Saccharomyces cerevisiae TISTR5606 was used for the fermentation. Ethanol was separated from the liquid hydrolysate by vacuum distillation at -80 kPa. Adsorption of the ethanol-water mixture was performed to increase the purity of ethanol. Optimal condition for the production of ethanol was also determined, with the ratio of S. cerevisiae (2, 4, 6, and 8 colonies) to 1 plate of T. reesei and cellulase enzyme (1.50 mL/g substrate) as the parameters. Optimal condition was noted when using 4 colonies of S. cerevisiae of to 1 plate of T. reesei. At such condition, ethanol at the concentration of 12.34% by volume was obtained. The vacuum distillation process was able to increase the ethanol concentration to 95.06% by volume. For adsorption of the ethanol-water mixture, molecular sieves types 3A and 4A were loaded at 190 g into a column; evaporation temperature of 80 °C, column temperature of 85 °C at 1 atm and the feed concentration of 95.06% by volume were used. When the feed flow rate was varied at 2.50 5.00 7.50 and 10.00 mL/min, the best condition was noted when using molecular sieve type 3A at a feed flow rate of 2.50 mL/min. The highest achievable ethanol concentration was 99.43% by volume. |
