Ethanol Production from Pineapple Waste by Co-culture of Saccharomyces cerevisiae TISTR 5339 and Candida shehatae KCCM 11422
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Creator 1. Waesarat Soontornchaiboon
2. Orawan Chunhachart
3. Ratchapol Pawongrat
Title Ethanol Production from Pineapple Waste by Co-culture of Saccharomyces cerevisiae TISTR 5339 and Candida shehatae KCCM 11422
Publisher Research and Technology Transfer Affairs Division
Publication Year 2559
Journal Title KKU Research Journal
Journal Vol. 21
Journal No. 37 (Supplement)
Page no. 347-355
Keyword Ethanol production,pineapple waste,ultrasonic,Saccharomyces cerevisiae TISTR 5339,Candida shehatae KCCM 11422
ISSN 0859-3957
Abstract The ethanol production from alkaline pretreated pineapple waste (core and peel) by using co-culture process of S. cerevisiae TISTR 5339 and C. shehatae KCCM 11422 was evaluated. The potential of ultrasound-assisted alkaline pretreatment was applied to enhance total reducing sugar concentration. The highest yield of total reducing sugar wasobtained at 21.84 g/g dried sample after pretreatment by 2% NaOH with ultrasonic-assistance for 60 min subsequently by enzymatic hydrolysis. The compositions of pretreated pineapple waste were 40.52?0.99% cellulose, 24.03?1.52% hemicellulose and 2.27?0.23% lignin. However, the dry matter loss reached to 44.67?3.21% after pretreatment. The structural changes of pretreated pineapple waste were observed by scanning electron microscopy (SEM). The maximum ethanol concentration and ethanol yield after 24 h fermentation were 9.73?0.15g/L and 0.45?0.01 g/g, respectively.
Asia-Pacific Journal of Science and Technology

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