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Screening of Bacillus spp. antagonistic bacteria for control bacterial fruit blotch disease and promote growth of watermelon seedling |
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| รหัสดีโอไอ | |
| Creator | Petcharat Thummabenjapone |
| Title | Screening of Bacillus spp. antagonistic bacteria for control bacterial fruit blotch disease and promote growth of watermelon seedling |
| Contributor | Titiya Khunwiangchan |
| Publisher | Faculty of Agriculture |
| Publication Year | 2568 |
| Journal Title | Khon Kaen Agriculture Journal |
| Journal Vol. | 53 |
| Journal No. | 6 |
| Page no. | 1279-1290 |
| Keyword | biological control, Acidovorax citrulli, bacterial fruit blotch, antagonistic, Bacillus spp., plant growth promoting bacteria |
| URL Website | https://li01.tci-thaijo.org/index.php/agkasetkaj |
| Website title | Khon Kaen Agriculture Journal |
| ISSN | 3027-6497 (Online) |
| Abstract | The objective of this research was to screen the antagonistic bacteria to control bacterial fruit blotch (BFB) caused by Acidovorax citrulli and promote the growth of watermelon seedlings. Eleven isolates of Bacillus spp. antagonistic i.e Bacillus-Ba029, Bacillus-Ba032, Bacillus-Ba033, Bacillus-Ba037N, Bacillus-BS, Bacillus-MS4, Bacillus-NTS3, Bacillus-PSK, Bacillus-S32, Bacillus-B1, Bacillus-K1 and Bacillus-K2 were selected for against 21 isolates of A. cirulli by dual culture bioassay. The results showed that 4 isolates (Bacillus-Ba029, Bacillus-Ba033, Bacillus-MS4 and Bacillus-NTS3) broadly inhibited the growth A. cirulli isolates to the maximum diameter of clear zone of 7.60 – 17.73 mm. The wettable powder bioproducts from selected 4 isolates of Bacillus were used to control BFB disease and promote plant growth compared by seed coating and control treatment in greenhouse conditions. The A. citrulli inoculated seeds and normal watermelon seeds were used. Results indicated that at 14 days after sowing, treatment using bioproducts Bacillus-Ba029, Bacillus-MS4 and Bacillus-Ba033 showed significantly decreased disease incidence as 14.50%, 17.00% and 18.25% and disease severity index as 29.25%, 29.95% and 32.75%, respectively. The control disease treatment with only A. citrulli showed 40% of disease incidence and 43.75%, respectively. In addition, these three isolates of Bacillus spp. were more effective in promoting plant growth as measured as plant height, flesh and dry weight of seedlings. Therefore, the Bacillus-Ba029, Bacillus-Ba033 and Bacillus-MS4 have high potential to develop bioproducts for biological control of bacterial fruit blotch disease and promote plant growth as well as reduce the percentage of seed-borne diseases in Cucurbitaceae. |
