|
Formulation and efficacy of alginate gel containing Steinernema siamkayai EPNKU70 against the tobacco cutworm, Spodoptera litura Fabricius and the beet armyworm, Spodoptera exigua Hübner |
|---|---|
| รหัสดีโอไอ | |
| Creator | Atirach Noosidum |
| Title | Formulation and efficacy of alginate gel containing Steinernema siamkayai EPNKU70 against the tobacco cutworm, Spodoptera litura Fabricius and the beet armyworm, Spodoptera exigua Hübner |
| Contributor | Chatchaloem Arkhan, Prakai Rajchanuwong, Apichat Vitta, Phanuruk Pratubkong, Kwanrudee Kachanan, Rattanawadee Onwong |
| Publisher | Faculty of Agriculture |
| Publication Year | 2568 |
| Journal Title | Khon Kaen Agriculture Journal |
| Journal Vol. | 53 |
| Journal No. | 6 |
| Page no. | 1222-1234 |
| Keyword | biological control, nematode application, noctuid moth, sodium alginate, infective juvenile |
| URL Website | https://li01.tci-thaijo.org/index.php/agkasetkaj |
| Website title | Khon Kaen Agriculture Journal |
| ISSN | 3027-6497 (Online) |
| Abstract | The tobacco cutworm (Spodoptera litura) and beet armyworm (S. exigua) are economically significant insect pests affecting various crops. Applying entomopathogenic nematodes (EPNs) is an effective biological control method against these pests. This study aims to develop an alginate gel formulation containing Steinernema siamkayai EPNKU70 for controlling S. litura and S. exigua larvae. Various formulations with different concentrations (0.5–2%) of sodium alginate and calcium chloride were evaluated for their ability to form spherical gel structures. Among these, formulations with 1.0–2.0% sodium alginate and calcium chloride successfully produced a spherical shape. An alginate gel formulated with a 1.0:1.0% ratio of sodium alginate to calcium chloride demonstrated high nematode persistence (93.23±2.80%) and survival (93.06±2.31%) after seven days. The alginate gel containing S. siamkayai EPNKU70 and supplemented with sugar as an attractant induced mortality in third-instar S. litura and S. exigua larvae at 88.33±9.83 and 81.66±14.72%, respectively, within 72 hours. These findings suggest that the alginate gel formulation of S. siamkayai EPNKU70 could serve as an effective alternative for managing S. litura and S. exigua larvae. |
