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Effect of stem cell secretory cytokines to chondrocyte progenitor cells |
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| รหัสดีโอไอ | |
| Creator | Supatra Klaymook |
| Title | Effect of stem cell secretory cytokines to chondrocyte progenitor cells |
| Contributor | Tatsanee Phermthai |
| Publisher | Nakhon Pathom Rajabhat University |
| Publication Year | 2561 |
| Journal Title | Journal of Thai Interdisciplinary Research |
| Journal Vol. | 13 |
| Journal No. | 5 |
| Page no. | 27 |
| Keyword | amniotic fluid stem cell, chondrocyte progenitor cell, secretory cytokine |
| URL Website | http://rdi.npru.ac.th |
| Website title | วารสารวิจัยสหวิทยาการไทย |
| ISSN | 2465-3837 |
| Abstract | According to previous studies, chondrocyte progenitor cells (CPC) found in human osteoarthritis (OA) cartilagehave been demonstrated for chondrogenic potentials and migratory capabilities. CPC have become promising cellsfor the treatment of OA. However, CPC cannot proliferate from traumatic cartilage, leading to unrepaired cartilageafter OA damage. Some studies reported for the effect of growth factors/ cytokines in stimulation of CPCoutgrowing from damaged cartilage. Secretory cytokines derived from stem cell culture was known as growthfactors/cytokines enrich. We hypothesized that secretory cytokines obtained from stem cell might stimulate theoutgrowing activity of CPC from OA cartilage.In this study, cartilage slices were co-cultured with secretory cytokines obtained from culture of amniotic fluidstem cells (AFS) and Wharton's jelly stem cells (WJSC) to compare with control medium. The period of celloutgrowth and numbers of outgrowing from cartilage tissues were observed under inverted microscope. Then,amount of outgrowing cells was assessed using MTT assay.The results demonstrated that cartilage tissues cultured under stem cell secretory cytokines displayed rapidoutgrowth. The number of outgrowing cells from cartilage tissues under the culture with AFS secretory factors washigher than the ones cultured under WJSC secretory cytokines (P = 0.0175) and control medium (P = 0.2992).Moreover, MTT assay also demonstrated that AFS secretory cytokines had the most effective among other studiesmedium (P < 0.05).We concluded that secretory cytokines in AFS culture medium has a high potential to stimulate CPC outgrowingfrom traumatic cartilage tissue in case of OA cartilage. |
