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Comparison of the immunosuppressive capacity of mesenchymal stem cellsderived from amniotic fluid, amniotic membrane and Wharton's jelly |
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| รหัสดีโอไอ | |
| Creator | Tatsanee Phermthai |
| Title | Comparison of the immunosuppressive capacity of mesenchymal stem cellsderived from amniotic fluid, amniotic membrane and Wharton's jelly |
| Contributor | Suparat Wichitwiengrat, Sasiprapa Thongbopit |
| Publisher | Nakhon Pathom Rajabhat University |
| Publication Year | 2561 |
| Journal Title | Journal of Thai Interdisciplinary Research |
| Journal Vol. | Vol. 13 |
| Journal No. | N0. 3 |
| Page no. | 11 |
| Keyword | amniotic fluid, amniotic membrane, immunosuppressive, peripheral blood mononuclear cell, Wharton's jelly |
| URL Website | www.rdi.npru.ac.th |
| Website title | วารสารวิจัยสหวิทยาการไทย |
| ISSN | 2465-3837 |
| Abstract | Mesenchymal stem cells (MSCs) have promise for clinical therapy due to their differentiation characteristic, invitro propagation ability and also low immunogenic property. MSCs have low expression of majorhistocompatability complex (MHC) class I and lack of MHC class II. However, MSCs derived from differentsources can exhibit differential immunosuppressive capacity. In this study, we investigated the immunosuppressivecapacity of MSCs derived from 3 different sources, including human amniotic fluid (AF), amniotic membrane (AM)and Wharton's jelly (WJ) by assessing the effect on phytohemagglutinin (PHA)-induced peripheral blood mononuclearcell (PBMC) proliferation. MSCs derived from AF (n = 6), AM (n = 10) and WJ (n = 10) were inactivated withmitomycin-C and co-cultured with PHA-induced PBMC in 1:10 ration for 72 hr. The MTT assay was used tocalculate the amount of PBMC proliferation, demonstrating immunosuppressive activity of MSCs. The co-culturedof these MSCs and PHA-induced PBMC was analyzed and showed into the percentage of the inhibition rate. After72 hr of co-culture, AF-MSCs, AM-MSCs and WJ-MSCs suppressed PHA-induced PBMC proliferation. WJ-MSCsshowed the highest of the percentage of inhibition rate at the 124.06% ? 24.76%, whereas AF-MSCs showed at the86.53% ? 8.81% (P value = 0.0033) and AM-MSCs showed 102.69% ? 19.2% (P value = 0.0448). Our resultshowed that WJ-MSCs had the stronger suppressive effect on PBMC among the 3 populations of MSCs in the cocultureexperiments. |
