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Leaf Constants and RAPD Marker of Jasminum multiflorum Cultivars Existing in Thailand |
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| รหัสดีโอไอ | |
| Creator | 1. Jindaporn Phithaksilp 2. Nijsiri Ruangrungsi 3. Kanchana Rungsihirunrat |
| Title | Leaf Constants and RAPD Marker of Jasminum multiflorum Cultivars Existing in Thailand |
| Publisher | Chulalongkorn University Press |
| Publication Year | 2559 |
| Journal Title | Journal of Health Research |
| Journal Vol. | 30 |
| Journal No. | 5 |
| Page no. | 329 |
| Keyword | Jasminum multiflorum, Cultivar, Leaf constants, RAPD fingerprint |
| ISSN | 8574421 |
| Abstract | Background: Medicinal plant identification is necessary for ensuring their efficacy and safety in quality control of herbal products. Methods:Four Jasminum multiflorumcultivars (Maluli, Maluli Sichomphu, Maluli Phum and Mali Ngachang) existing in Thailand was first studied for their leaf constants (stomatal number, stomatal index, epidermal cell number, epidermal cell area, palisade ratio, vein termination, vein islet number and trichome number) and random amplified polymorphic DNA (RAPD) fingerprint.Results: Leafconstantsanalysis demonstrated thattrichome number was useful to identify these 4 J. multiflorumcultivars. According to the present of trichome on adaxial surface of leaf, J. multiflorum caltivar Maluli Phum can be distinguished. Among the rest 3 cultivars, the number of trichome on abaxial surface (17.49 ฑ 3.66) describes J. multiflorumcultivar Mali Ngachang. J. multiflorumcultivar Maluli and Maluli Sichomphu can be characterized by vein termination number. Leaf constants evaluation is proved to be one of the methods capable in plant authentication even among cultivar. For RAPD analysis among 4 J. multiflorumcultivars, dice similarity index ranged from 0.2583-0.7922. The cluster diagram could be divided into two clusters. Cluster I includes 2 cultivars of J. multiflorumcultivar Maluli Sichomphu and Maluli showing 0.7922 similarity index and cluster II includes 2 cultivars of J. multiflorumcultivar Maluli Phum and Mali Ngachang showing 0.4222 similarity index.Conclusions:Leaf constants (stomatalnumber, stomatal index, epidermal cell number, epidermal cell area, palisade ratio, vein termination, vein islet number and trichome number) and RAPD fingerprintsare useful techniques for plantcultivaridentification |
