|
Peanut hairy root culture extract suppresses migration and invasion of highly metastatic cholangiocarcinoma cells |
|---|---|
| รหัสดีโอไอ | |
| Creator | Suchada Phimsen |
| Title | Peanut hairy root culture extract suppresses migration and invasion of highly metastatic cholangiocarcinoma cells |
| Contributor | Piyathida Wongkham, Donruetai Thalapphet, Warissara Wongkham, Apinun Limmongkon |
| Publisher | The Pharmacological and Therapeutic Society of Thailand |
| Publication Year | 2569 |
| Journal Title | Journal of Basic and Applied Pharmacology |
| Journal Vol. | 6 |
| Journal No. | 2 |
| Page no. | O77-88 |
| Keyword | cholangiocarcinoma, peanut hairy root culture extract, prenylated stilbenoids, cell migration, cell invasion |
| URL Website | https://li01.tci-thaijo.org/index.php/JBAP |
| Website title | เว็บไซต์คลังข้อมูล |
| ISSN | 2774-0854 (Online) |
| Abstract | Cholangiocarcinoma (CCA) is a highly aggressive malignancy withpoor prognosis, partly because of late-stage diagnosis, therapeutic resistance, and metastatic progression.This study investigated the anticancer and anti-migratory, and anti-invasive effects of peanut hairy root culture crude extract (PCE), a source of prenylatedstilbenoidsincluding trans-arachidin-1andtrans-arachidin-3. The effectsof PCE on cell viability wereinitially screened inKKU-213B, A549, MCF-7, and KBcancer cells. PCE reduced cell viability in a concentration-dependent manner, with KKU-213Bcells showing the greatest sensitivity among the tested cell lines. The effects of PCE on cell viabilitywere subsequently evaluatedin a panel of CCA cell linescomprisingKKU-213A, KKU-213B, KKU-055, and the highly metastatic KKU-213AL5subline.KKU-213AL5 cellsexhibited the greatest sensitivity and were therefore selected for further investigation. At non-cytotoxic concentrations, PCE significantly suppressed KKU-213AL5 cell migration andinvasion, as determined by wound-healing and Matrigel-coated Transwell assays, respectively. Gelatin zymography showedthat PCE did not significantly alterMMP-2orMMP-9 activity. Collectively, these findings demonstrate that PCE inhibitsthe cell viability, migration, and invasion of highly metastatic CCA cells,supportingfurther investigation of its active constituentsand underlying molecular mechanism. |
