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Effect of the XmnI-Gg polymorphism on HbE and red blood cell parameters of Hb E carriers with and without SEA-? thalassemia 1 |
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| รหัสดีโอไอ | |
| Creator | 1. Prapapun Leckngam 2. Eakthong Limweeraprajak 3. Tiemjan Keawkarnkha |
| Title | Effect of the XmnI-Gg polymorphism on HbE and red blood cell parameters of Hb E carriers with and without SEA-? thalassemia 1 |
| Publisher | Faculty of Associated Medical Sciences, Chiang Mai University |
| Publication Year | 2562 |
| Journal Title | Journal of Associated Medical Sciences |
| Journal Vol. | 52 |
| Journal No. | 3 |
| Page no. | 199-204 |
| Keyword | HbE carrier, SEA-? thalassemia 1 carrier, XmnI-G? polymorphism, RBC indices |
| URL Website | https://www.tci-thaijo.org/index.php/bulletinAMS/index |
| Website title | Journal of Associated Medical Sciences |
| ISSN | 25396056 |
| Abstract | Backgroud: XmnI-Gg polymorphism has been found to be the major cis-acting factor responsible to increased g-globin gene activation and closely linked to HbE. It was expected that this polymorphism might modify the HbE and RBC indices in HbE carriers. Objectives: To determine the effect of the XmnI-Gg polymorphism on RBC indices and HbE levels in single HbE carriers and double HbE/SEA-a thalassemia 1 carriers. Materials and methods: The studied samples comprised 160 EDTA blood collected from routine Hemoglobin Typing Laboratories of Swan Pracharak Hospital and Lampang Regional Hospital. The XmnI-Gg polymorphism was determined by the PCR-RFLP analysis. The SEA-a thalassemia 1 was genotyped by Gap-PCR. HbE level was determined by cation-exchange HPLC, and RBC indices by automated hematology analyzer. Mann-Whitney U test was computed to analyze the data with p-value of less than 0.05 considered to be statistically significant. Results: The prevalence of the XmnI-Gg (+/+) was 13.9% and 6.3%, of the XmnI-Gg (+/-) was 66.0%, 81.3%, and of the XmnI-Gg (-/-) was 20.1% and 12.5% in the single HbE carriers and double HbE/SEA-a thalassemia carriers, respectively. Presence of the XmnI-Gg site did not affect HbE and RBC indices in both single HbE carriers and double HbE/SEA-a thalassemia 1 carriers. Conclusion: XmnI-Gg polymorphism did not effect the RBC indices and HbE levels in HbE carriers. It was not the confounding factor to be concerned when considering RBC parameters in screening for HbE/SEA-a thalassemia 1 double carriers and can be ignored. |
