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Design of Oligonucleotide Primers for the Development of a Method for Typing Salmonella Isolates in the Russian Federation |
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| รหัสดีโอไอ | |
| Creator | Irina Soltynskaya, Alexandra Bogomazova, Ekaterina Krylova, Olga Prasolova, Olga Ivanova |
| Title | Design of Oligonucleotide Primers for the Development of a Method for Typing Salmonella Isolates in the Russian Federation |
| Contributor | - |
| Publisher | TuEngr Group |
| Publication Year | 2565 |
| Journal Title | International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies |
| Journal Vol. | 13 |
| Journal No. | 12 |
| Page no. | 13A12T: 1-7 |
| Keyword | Salmonella Enterica, DNA sequencing, bacterial typing, serovar, CRISPR 1 locus |
| URL Website | http://TuEngr.com/Vol13-12.html |
| Website title | ITJEMAST V13(12) 2022 @ TuEngr.com |
| ISSN | 2228-9860 |
| Abstract | This article presents the results of work on the creation of molecular genetic methods for the control of industrial and vaccine strains of microorganisms, including methods for typing salmonellosis pathogens to serovar. The technique is based on the sequence analysis of a fragment of a highly polymorphic region of the CRISPR1 locus by Sanger DNA sequencing. Using the reference genomes of Salmonella enterica from databases and our own whole genome sequencing data, we designed oligonucleotide primers and assessed their quality and specificity. |
