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An Application of Loop Isothermal Amplification to Detect African Swine Fever Virus |
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| รหัสดีโอไอ | |
| Creator | Larisa Gnezdilova, Sergey Pozyabin, Seidfatima Borunova, Tatyana Stoyan, Marina Selina, Ekaterina Davydova |
| Title | An Application of Loop Isothermal Amplification to Detect African Swine Fever Virus |
| Contributor | - |
| Publisher | TuEngr Group |
| Publication Year | 2565 |
| Journal Title | International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies |
| Journal Vol. | 13 |
| Journal No. | 9 |
| Page no. | 13A9I: 1-9 |
| Keyword | Loop-mediated isothermal amplification, LAMP, Virus ASF, PCR diagnostics. |
| URL Website | http://TuEngr.com/Vol13-9.html |
| Website title | ITJEMAST V13(9) 2022 @ TuEngr.com |
| ISSN | 2228-9860 |
| Abstract | Loop isothermal amplification (LAMP) is an alternative to PCR that reduces the time of analysis and does not require the use of expensive equipment. Here, we describe our real-time LAMP technique with hybridization fluorescence detection for the detection of ASF virus in biological material from animals. The detection limit of the technique is 2*103 copies/ml, which is comparable to the sensitivity of PCR. The high specificity of the method developed by LAMP was shown, and no false positive results were observed when testing other viruses and microorganisms, as well as biological samples that did not contain DNA of the ASF virus. In comparative tests on blood plasma samples (n=33) and a suspension of pathological material of the tonsils, spleen, lungs (n=30), the results of PCR and LAMP coincided almost completely, with the exception of one LAMP false-negative sample with a low viral load (PCR Ct ~ 38). |
