|
GENETIC DISTANCES AMONG EIGHT ORNAMENTAL CHICKENS BY MOLECULAR TECHNIQUE |
|---|---|
| รหัสดีโอไอ | |
| Creator | Yousif M.S. Al-Barzinji, Mohsin A. Ahmad, Bakhtiyar Khaild Saeed |
| Title | GENETIC DISTANCES AMONG EIGHT ORNAMENTAL CHICKENS BY MOLECULAR TECHNIQUE |
| Contributor | - |
| Publisher | TuEngr Group |
| Publication Year | 2563 |
| Journal Title | International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies |
| Journal Vol. | 11 |
| Journal No. | 2 |
| Page no. | 11A02P: 1-10 |
| Keyword | Ornamental chicken, Mitochondrial DNA, RAPD-PCR, DNA locus, RAPD primer, Mitochondrial Cytochrome b, Genetic Distance test. |
| URL Website | http://TuEngr.com/Vol11_2.html |
| Website title | ITJEMAST V11(2) 2020 @ TuEngr.com |
| ISSN | 2228-9860 |
| Abstract | The aim present study is to determine genetic distances among three local ornamentals with five commercial ornamental chickens using ten RAPD primer and specific mitochondrial DNA locus. Twenty RAPD primers were used; ten out of them were selected based on their number of bands (NB) and polymorphic characteristics. A total of 66 bands observed, 55 of them are polymorphic bands among chicken breeds, whereas overall bans 13 of them are unique bands whose highest unique band was obtained in primer OPA-20 and OPM-06. RAPD-PCR dendrograms show that the distance among ornamental chicken arrived 50.29% and phylogenetic dendrograms showed that two clusters, the first cluster branch consisted black and black-white local ornamental chicken, Silver Polish and Germany Phoenix Bantam with 25.59% distance between them while the second cluster also including local black-barred, American white brahman, Sultan Belgian, and American Lamborghini with 17.83 % genetic distance between them. The mitochondrial DNA dendrograms also show two main clusters among local and commercial ornamental chickens, the local black and black-white are in the first cluster while the local black-barred located at other clusters. It was concluded that the black, black-white was closer to each other. The high genetic distance 55.3% among breeds and variation in phenotypic shapes with different colors for ornamental chickens indicates that these local ornamental chickens have a good amount of genetic resources to made genetically improvement in further and it means the three local ornamental chickens are independent breeds. |
