|
Induction of Somatic Embryogenesis in Torenia fournieri Lind. |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. Nattapong Chanchula 2. Thunya Taychasinpitak 3. Anchalee Jala 4. Theerachai Thanananta 5. Shinji Kikuchi |
| Title | Induction of Somatic Embryogenesis in Torenia fournieri Lind. |
| Publisher | TUENGR GROUP |
| Publication Year | 2558 |
| Journal Title | International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies |
| Journal Vol. | 6 |
| Journal No. | 4 |
| Page no. | 165 |
| Keyword | embryogenic callus, tissue culture, young leaves, picloram, in vitro |
| ISSN | 2228-9860 |
| Abstract | Somatic embryogenesis is one biotechnological method that can be used to produce genetically identical plants for use in plant breeding. In this research, we attempted to induce the formation of embryogenic callus from young leaves excised from in vitro Torenia spp. by culturing in the dark for 1, 2, 3 and 4 weeks on Murashige and Skoog (MS) medium with Picloram solution added at the concentrations of 0.5, 1, 1.5 and 2 mg-1 and 2,4-D solution added at the concentrations of 0.5, 1, 1.5 and 2 mg-1. The leaf explants from all the 2,4-D treatments turned brown and died, but the leaf explants cultured on media with Picloram added at every concentration tested formed soft, loosely aggregated callus tissue. Callus tissue was induced from leaves of diploid Torenia to the greatest degree (95% callus formation at 3 weeks) when cultured on MS medium containing Picloram at 1.5 mg-1 in the dark, and callus tissue tended to turn brown with increasing Picloram concentration and increasing incubation time. For polyploid Torenia, the highest percentage of callus formation was observed on leaves cultured on MS medium containing Picloram, and callus tissue also tended to turn brown with increasing Picloram concentration and increasing incubation time. Following transfer of the embryogenic callus tissue to hormone-free MS medium under light conditions (16-h photoperiod), the greatest rate of somatic embryo formation was observed, in case of both diploid and polyploid Torenia accessions. |
