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Introduction of Astaxanthin Biosynthesis Gene crtW into Petunia and Calibrachoa Using Agrobacterium-mediated Transformation |
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| รหัสดีโอไอ | |
| Creator | 1. Bodin Phadungsawat 2. Thunya Taychasinpitak 3. Shermarl Wongchaochant 4. Sakae Suzuki |
| Title | Introduction of Astaxanthin Biosynthesis Gene crtW into Petunia and Calibrachoa Using Agrobacterium-mediated Transformation |
| Publisher | TUENGR GROUP |
| Publication Year | 2558 |
| Journal Title | International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies |
| Journal Vol. | 6 |
| Journal No. | 5 |
| Page no. | 235 |
| Keyword | Carotenoid, Genetic engineering, Brevundimonas sp., EHA105 |
| ISSN | 2228-9860 |
| Abstract | Agrobacterium-mediated gene transfer method for transferring crtW gene was investigated in Petunia and Calibrachoa by using Agrobacterium tumefaciens strain EHA105, harboring a plasmid containing the crtW gene from Brevundimonas sp. strain SD212 under 35S promoter with kanamycin resistant gene for selection. Co-cultivation was done using leaf disk method for 3 days in Agrobacterium solution with 20 ppm acetosyringone and selection was carried out on selective MS medium containing 200 ppm kanamycin. Inoculated leaf explants of Petunia and Calibrachoa produced putative transgenic callus tissue that demonstrated orange color and astaxanthin accumulation. The result of PCR analysis indicated the Petunia and Calibrachoa calluses contained the crtW gene and RT-PCR result showed crtW gene expression in the callus tissue. |
