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Effect of phenol red on cell cultures |
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| รหัสดีโอไอ | |
| Creator | Nathaphon Kuncharoenwirat |
| Title | Effect of phenol red on cell cultures |
| Contributor | Waranya Chatuphonprasert, Kanokwan Jarukamjorn |
| Publisher | Faculty of Pharmaceutical Sciences KKU, MSU, UBU |
| Publication Year | 2564 |
| Journal Title | Isan Journal ofPharmaceutical Sciences |
| Journal Vol. | 17 |
| Journal No. | 1 |
| Page no. | 13-23 |
| Keyword | phenol red, cell culture, estrogenic effect, cell proliferation, in vitro model |
| URL Website | https://tci-thaijo.org/index.php/IJPS |
| Website title | Isan Journal ofPharmaceutical Sciences,IJPS |
| ISSN | 19050852 |
| Abstract | Cell culture is an in vitro model for primary screening of the pharmacological and toxicological potential of a substance before in vivo and clinical studies. Phenol red is the pH indicator in culture medium indicating a suitable time for medium change. Phenol red has estrogenic activity due to its non-steroidal estrogenic hormone-structure like 17b-estradiol and bisphenol. Phenol red-free medium is sometimes employed for culturing and treatment periods to avoid undesirable effects, e.g. interference with phase I and phase II enzyme activities, estrogenic activity, stimulating cell proliferation and differentiation, decreasing pharmacotherapeutic activity of anti-cancer drugs, and enhancing activity of anabolic compounds, particularly in estrogen-sensitive cells such as breast cancer cells and ovarian cells. Phenol red shows variable effects that can depend on its concentration and the type of cell, although relevant evidence is limited. Therefore, using phenol red-contained or -free medium during cell culture or treatment is a crucial choice for experimental design in order to prevent unreliable outcomes. |
