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Formulation and characterization of cream containing Yang-na oleoresin-loaded in polymeric nanoparticles |
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| รหัสดีโอไอ | |
| Creator | Toum Lathsamee |
| Title | Formulation and characterization of cream containing Yang-na oleoresin-loaded in polymeric nanoparticles |
| Contributor | 1)Watcharee Khunkitti 2)Duangkamon Sakloetsakun |
| Publisher | Faculty of Pharmaceutical Sciences KKU MSU UBU |
| Publication Year | 2562 |
| Journal Title | Isan Journal ofPharmaceutical Sciences |
| Journal Vol. | 15 |
| Journal No. | 1 |
| Page no. | 166-180 |
| Keyword | Formulation and characterization of cream containing Yang-na oleoresin-loaded in polymeric nanoparticles |
| URL Website | https://tci-thaijo.org/index.php/IJPS |
| Website title | Isan Journal ofPharmaceutical Sciences; IJPS |
| ISSN | 19050852 |
| Abstract | Introduction: The aims of this study were to formulate and characterize formulations consisting Yang-na oleoresin-loaded in polymeric nanoparticles. Yang-na oleoresin consisted of essential oil and phenolic compounds which are sensitive to light, air and oxygen. To overcome this obstacle, Yang-na oleoresin was prepared by loading in polymeric nanoparticles before incorporate into creams. Materials and methods: Firstly, physicochemical properties of Yang-na oleoresin, for instance, miscibility, total phenolic compounds (TPC) and antioxidant activities were investigated. Secondly, Yang-na oleoresin was loaded in polymeric nanoparticles using chitosan and gellan gum by complex-coacervation method. The chitosan-gellan gum (CS-GG) nanoparticles-loaded Yang-na oleoresin were characterized in terms of zeta potential, particle size and %entrapment efficiency. The suitable formulation was selected to incorporate into oil in water (o/w) cream base. Lastly, physicochemical characteristics of creams including appearance, odor, pH-value, viscosity, phase separation and amount of TPC were investigated both before and after accelerated stability. The accelerated stability was conducted by heating/cooling for 6 cycles. Results: Results of miscibility study revealed that Yang-na oleoresin was well dissolved in absolute ethanol and dimethyl sulfoxide in the ratio of 1:50. The amount of TPC of 2% (v/v) of Yang-na oleoresin in absolute ethanol was 1.683 ? 0.057 mgGAE/ml. The 50%inhibitory concentration of Yang-na oleoresin was 0.931 mg/ml determined by DPPH assay whereas the %lipid peroxidation of Yang-na oleoresin was 83.51%. The particle size, zeta potential and %entrapment efficiency of optimized CS-GG nanoparticles-loaded with Yang-na oleoresin were 128 nm, 14 mV and 27.82%, respectively. After accelerated stability, cream base, control cream and Yang-na cream preparing from the suitable formulation which consisted of glycerol monostearate had smooth texture and showed the highest stability. The pH-value and the viscosity of these formulations were 4.6-5.4 and 22.745 ? 463-29,337 ? 522 cP, respectively. The amounts of TPC containing in the Yang-na cream and control cream were found to be 93.6 and 80.1%, respectively. The amount of TPC in Yang-na cream was significantly higher than the control cream (p=0.036). Conclusion: It was concluded that the formulation of cream containing Yang-na oleoresin-loaded in polymeric nanoparticles had the highest stability compared to other formulations and it was suitable for human skin. Furthermore, the polymeric nanoparticle system was proven to be able to protect the active compounds against oxidation process. |
