|
Comparison of human and porcine skins as the barrier membraneinan in vitro permeation of plant polyphenols |
|---|---|
| รหัสดีโอไอ | |
| Creator | Aroonsri Priprem |
| Title | Comparison of human and porcine skins as the barrier membraneinan in vitro permeation of plant polyphenols |
| Contributor | Weeraya Sukkhamduang, Weerawat Teeranachaideekul, Supatra Porasuphatana, Teerasak Damrongrungruang, Malyn Chulasiri |
| Publisher | Faculty of Pharmaceutical Sciences KKU MSU UBU |
| Publication Year | 2554 |
| Journal Title | Isan Journal ofPharmaceutical Sciences |
| Journal Vol. | 7 |
| Journal No. | 3 |
| Page no. | 39-46 |
| Keyword | In vitro permeation, phenolic compounds, plant extracts, porcine skin and human skin |
| URL Website | https://tci-thaijo.org/index.php/IJPS |
| Website title | Isan Journal ofPharmaceutical Sciences, IJPS |
| ISSN | 19050852 |
| Abstract | Introduction: Porcine and human skin were compared as barrier membranes in in vitro permeation of polyphenols from plant extracts as sources of antioxidants to be used as value-added ingredients in novel cosmetic products. In vitro permeation was intended to assess expected release of ingredients from the applied products. Materials and Method: Excised skins from porcine ears and human abdomen were used as the barrier membrane in diffusion cells for comparison of polyphenols from 7 plant extracts; dahla (EE), damask rose(RD), buaput (RK), samohtai (TC), samohpipek (TB), blue butterfy pea (CT) and red rose (RH) at 32 oC. Total polyphenols were analyzed by Folin-Ciocalteaucolorimetry at 765 nm using gallic acid as the standard and permeation rates were statistically compared. Results: Donor concentrations of EE, RD, RK, TC, TB, CT and RH were 5, 13.5, 56, 42, 37, 3.6 and 8.9 mg GAE?ml-1. Relationships between donor polyphenol concentrations and permeation rates using porcine skin showed a linear correlation (r = 0.826) but not when using the human skin as the barrier (r = 0.321). There were lag time of about 12 22 h when using the human skin but not porcine. Thus, porcine and the human skin did not provide the same permeation profles which included rate, cumulative amount and lag time. Conclusion: Porcine skin cannot be used as a substitute of the human skin in in vitro permeation of plant extracts with substantially low quantities of polyphenols. |
