|
Anticancer Protein from Bitter Melon Cultivated in Thailand |
|---|---|
| รหัสดีโอไอ | |
| Creator | Rith Watthanachaiyingcharoen |
| Title | Anticancer Protein from Bitter Melon Cultivated in Thailand |
| Contributor | Wichet Leelamanita |
| Publisher | Faculty of Pharmaceutical Sciences KKU MSU UBU |
| Publication Year | 2552 |
| Journal Title | Isan Journal ofPharmaceutical Sciences |
| Journal Vol. | 5 |
| Journal No. | 1 |
| Page no. | 82-90 |
| Keyword | Bitter melon, Momordica charantia, Apoptosis, Anticancer protein |
| URL Website | https://tci-thaijo.org/index.php/IJPS |
| Website title | Isan Journal ofPharmaceutical Sciences, IJPS |
| ISSN | 19050852 |
| Abstract | Protein extraction from bitter melon (Momordica charantia L.) cultivated in Thailand composed of amount of different proteins. The major protein was 30 kDa in size. For this study, we purified the proteins by using Resource S (ion exchange column) and following by Superdex 200 HR10/30 (gel filtration) equipped with an FPLC apparatus. They were glycoproteins with Isoelectric point (pI) > 8.4 and molecular weights of approximately 30 kDa. Both possessed potent anticancer activity against L929 (mouse fibroblasts) at the IC?? of 1.7 ?g/ml possibly via apoptotic pathways determined by DNA fragmentation and cells' morphology change methods. |
