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Effects of Bisphenol A on the Expression of CYP1A Transcripts in Juvenile False Clown Anemonefish (Amphiprion ocellaris) |
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| รหัสดีโอไอ | |
| Creator | Atittaya Khamkaew, Jeeranan Thamnawasolos, Chuta Boonphakdee, Thanomsak Boonphakdee, Karnjana Hrimpeng |
| Title | Effects of Bisphenol A on the Expression of CYP1A Transcripts in Juvenile False Clown Anemonefish (Amphiprion ocellaris) |
| Contributor | Atittaya Khamkaew, Jeeranan Thamnawasolos, Chuta Boonphakdee, Thanomsak Boonphakdee, Karnjana Hrimpeng |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2563 |
| Journal Title | Genomics and Genetics |
| Journal Vol. | 13 |
| Journal No. | 2&3 |
| Page no. | 69-78 |
| Keyword | biomarker, BPA, clownfish, cyp1a, qRT-PCR |
| URL Website | https://li01.tci-thaijo.org/index.php/gst/issue/view/16872 |
| Website title | https://li01.tci-thaijo.org/index.php/gst/article/view/244927 |
| ISSN | 24655198 |
| Abstract | Bisphenol A (BPA), used in the manufacturingof various plastic products, iswidely distributed in the marine environment and significantly impactsaquatic wildlife. In this study, juvenile false clown anemonefish(Amphiprion ocellaris)was used as a model species for low-dose BPA exposure,and to examinethe cytochrome P450 1Agene(cyp1a)as a potential biomarker. Fish wereexposed to BPA at a range of concentrationsfor up to 48 hours.Quantification of BPAin the exposed animals revealed that accumulationwassignificantlyhigher in the liver than in the muscle. Therefore, liver tissue was selected for further study.In addition to this, the selection of an appropriatereference gene for quantitative real-time reverse transcription-polymerasechain reaction (qRT-PCR) was investigated.Ourresultsshowed thatribosomal proteinl8gene (rp18) was the most stable reference gene,compared to 18S ribosomal RNAgene(18SrRNA)and elongation factor 1?gene(EF-1?). This was used as an internal control in the qRT-PCR experiments. Following exposureto BPA, cyp1aexpression significantly increasedin a dose-and time-dependent manner:after 24 hours exposure to 50 ng/l BPA, it increased2.82-fold; and after 12 hours exposure to 100 ng/l BPA, it increased 3.44-fold. A lowdose of BPA rapidlyinducescyp1aexpressionin this sentinel species. Thus, cyp1acould be usedas apotential biomarker for BPAexposure. |
