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Expression analysis and nucleotide variation of OsC1 gene associated with anthocyanin pigmentation in rice in Thai rice cultivars. |
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| รหัสดีโอไอ | |
| Creator | Lalita Na Rachasima |
| Title | Expression analysis and nucleotide variation of OsC1 gene associated with anthocyanin pigmentation in rice in Thai rice cultivars. |
| Contributor | Lalita Na Rachasima, Roypim Sukkasem, Saengtong Pongjaroenkit, Varaporn Sangtong, Srimek Chowpongpang and Chotipa Sakulsingharoj |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2560 |
| Journal Title | Genomics and Genetics |
| Journal Vol. | 10 |
| Journal No. | 3 |
| Page no. | 46-53 |
| Keyword | OsC1 gene, rice, anthocyanins, transcription factor |
| URL Website | https://www.tci-thaijo.org/index.php/gst/issue/view/9399 |
| Website title | https://www.tci-thaijo.org/index.php/gst/article/view/86262 |
| ISSN | 24655198 |
| Abstract | OsC1 gene, encoding a R2R3-Myb transcription factor, regulates expression of structural genes in anthocyanins biosynthesis pathway in rice. It plays an important role in rice coloration. Expression of this gene in both leaf and seed tissues was examined in three rice cultivars covering white (Khao Dawk Mali 105; 105), red (Sukhothai Hom Dang; SKHD) and black (Kham; KN) rice. Expression of the OsC1 gene was found in young leaves and seeds in all rice cultivars in this study. Higher level of gene expression was observed in seed tissues of Kham black rice at 25 days after flowering, associated with dark anthocyanin pigmentation on the pericarps. However, the expression patterns of this gene in leaves and seeds in white and red rice were stable and appeared at similar level, not corresponding with the phenotypic traits of red rice color at the late stage of development compared with the white one. Comparisons of nucleotide sequences in the exon 3 of the OsC1 gene among those three cultivars revealed a 10-bp deletion in white and red rice, resulting in frameshift mutation in R3 region of Myb DNA binding domain, but not in the black. This deletion caused the functional loss in white (105) rice, leading to their anthocyanin pigment accumulation loss at all stages and tissue types. For red (SKHD) rice, although the expression of OsC1 was detected at all stages, its similar 10-bp deletion in exon 3 of the OsC1 gene indicated an alternative pathway for anthocyanin pigment to be accumulated in the pericarp tissues. The result from this study constitute a basis on how pigment would be developed in these three Thai rice cultivars and are applied to rice improvement by developing molecular markers for selection of rice anthocyanin pigmentation in future. |
