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Heat shock protein 70 gene polymorphism in KU-Phupan black-bone chicken |
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| รหัสดีโอไอ | |
| Creator | Piyamas Phongkaew |
| Title | Heat shock protein 70 gene polymorphism in KU-Phupan black-bone chicken |
| Contributor | Piyamas Phongkaew, Panuwat Khumpeerawat |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2560 |
| Journal Title | Genomics and Genetics |
| Journal Vol. | 10 |
| Journal No. | 1&2 |
| Page no. | 7-12 |
| Keyword | heat shock protein 70 gene, heat stress, KUPhuphan blackbone chicken, PCRRFLP |
| URL Website | https://www.tci-thaijo.org/index.php/gst/issue/view/7723 |
| Website title | https://www.tci-thaijo.org/index.php/gst/article/view/67818 |
| ISSN | 24655198 |
| Abstract | The objective of this study was to investigate the polymorphism of heat shock protein 70 (hsp70) gene in the second generation of KUPhuphan black-bone chicken populations comparing with crossbreed native chicken and commercial layer chicken populations. Thirty, twentyfive, twentythree and twentythree samples of the second generation of KUPhuphan blackbone female chickens (FB) and male chickens (MB), crossbreed native chickens (BR), and the layer commercial line (LA) were genotyped hsp70 gene by the PCRRFLP technique. The hsp70 gene showed two loci, locus C generated by EaeI and locus M created by MmeI. The locus C consisted of two alleles (C1 and C2) providing three genotypes of C1C1, C1C2 and C2C2. The allele and genotype frequencies of KUPhuphan black-bone chicken populations were not different from other Thai indigenous breeds. The locus M consisted of two alleles (M1 and M2) whereas the two genotypes of M1M2 and M2M2 were observed. The allele M1 showed low allele frequency and was only found in the MB population. The allele M2 showed monomorphism in FB, BR and LA populations. The male KUPhuphan black-bone chickens exhibited different M1M2 genotype from other detected populations but the allele frequency of MB was less than other Thai indigenous chickens. The genotype frequency of the candidate thermotolerance genotype C1C2/M2M2 of both KUPhuphan black-bone chicken populations was higher than other genotypes. According to the results, KUPhuphan black-bone chickens showed variation in genotypes, especially for locus C, and contained high genotype frequency of the candidate thermotolerance genotype. Therefore, the locus C and the candidate genotype of hsp70 gene could be used as a genetic marker related with heat tolerance for selection and the breeding program of KUPhuphan black-bone chickens to create heat tolerance chickens. |
