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Effects of some psychiatric drugs on corticotropin-releasing factor (CRF) gene expression in mouse hypothalamus cells in vitro |
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| รหัสดีโอไอ | |
| Creator | Matchima Nantharat, Rachaneekorn Tammachote, Verayuth Praphanphoj |
| Title | Effects of some psychiatric drugs on corticotropin-releasing factor (CRF) gene expression in mouse hypothalamus cells in vitro |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2559 |
| Journal Title | Genomics and Genetics |
| Journal Vol. | 9 |
| Journal No. | 1 |
| Page no. | 19 - 24 |
| Keyword | Psychiatric drugs, HPA, CRF |
| ISSN | 24655198 |
| Abstract | Psychiatric drugs are typically used to treat patients suffered from mental disorders and some conditions of stress-related disorders. Effects of psychiatric drugs actions were proposed to be involved in regulation of neuronal gene expression. One of the genes involved is corticotropin-releasing factor (CRF) gene that plays a critical role in controlling the hypothalamus-pituitary-adrenal (HPA) axis under stress condition. This study aims to examine the effects of psychiatric drugs on CRF gene expression in a mouse hypothalamus cell line. Quantitative real-time PCR was used to determine expression levels of CRF gene in cells treated with diazepam, risperidone and haloperidol. Results showed that all of the drugs tested tend to suppress CRF gene expression at the RNA levels, both before and after the cells were incubated with forskolin, a stress-inducing agent. The findings suggested that the mechanism of psychiatric drugs actions may act in HPA negative-feedback control by regulating CRF mRNA expression in response to stress. |
