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Potential application of immobilized Bacillus subtilis (P5-6) as bio-protective culture against Staphylococcus aureus in acidic and salted food model |
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| รหัสดีโอไอ | |
| Creator | Vishal Chhetri |
| Title | Potential application of immobilized Bacillus subtilis (P5-6) as bio-protective culture against Staphylococcus aureus in acidic and salted food model |
| Contributor | Natthanan Buranavanitvong, Sarn Settachaimongkon, Nattida Chotechuang, Cheunjit Prakitchaiwattana |
| Publisher | Mahasarakham University |
| Publication Year | 2566 |
| Journal Title | Food Agricultural Sciences and Technology (FAST) |
| Journal Vol. | 9 |
| Journal No. | 1 |
| Page no. | No. 66-75 |
| Keyword | Bacteriocin, Bacillus, protective culture, gene expression, S. Aureus |
| URL Website | https://ph02.tci-thaijo.org/index.php/stej/index |
| Website title | Food Agricultural Sciences and Technology (FAST) |
| ISSN | 2822-1001 (Print), 2822-101X (Online) |
| Abstract | This research is aimed to apply bacteriocin-producing halophilic bacteria from salty fermented foods, B. subtilis (P5-6) against S. aureus as bio-control agent in bamboo shoot pickle. Expression of genes associated with bacteriocin activity of the B. subtilis (P5-6) was investigated. Only subtilin gene expressions were observed along with housekeeping gene This research aimed to apply bacteriocin producing strain Bacillus subtilis (P5-6) as protective culture against Staphylococcus aureus in bamboo shoot pickle. Expression of genes associated with bacteriocin activity of the B. subtilis (P5-6) was investigated. Only subtilin gene expressions were observed along with housekeeping gene BA-rpoB when co-cultured with S. aureus. B. subtilis (P5-6) protective culture was made in a simple form as dried mango pieces containing 2.5 mg/g salt with viable count at 6.25 log CFU/g and water activity (aw) at 0.49, respectively. The Protective culture was made by immobilizing cells and culture of P5-6 in freeze-dried mango pieces and drying with two-step drying process. The Minimum Inhibitory Concentration (MIC) of the protective culture was 20 AU/mg and Minimum Bactericidal Concentration (MBC) at 80 AU/mg. When applied as bio-preservative in bamboo shoot pickle, the protective culture significantly (p<0.05) reduced the level of S. aureus contamination and had no impact on this food properties. This study demonstrated the potential of P5-6 in this simple protective culture form in controlling the growth of S. aureus, therefore it could be a potential for further development as bio-preservative to enhanced safety of food products. |
