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Microbial Production of Ascorbic Acid from Brewery Spent Grain (BSG) by Aspergillus flavus andAspergillus tamarii |
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| รหัสดีโอไอ | |
| Creator | Temitope Banjo, Sarafadeen Kareem , Temitope Popoola and Oluseyi Akinloye |
| Title | Microbial Production of Ascorbic Acid from Brewery Spent Grain (BSG) by Aspergillus flavus andAspergillus tamarii |
| Publisher | Faculty of Agro-Industry |
| Publication Year | 2561 |
| Journal Title | Food and Applied Bioscience |
| Journal Vol. | 6 |
| Journal No. | 2 |
| Page no. | 93-105 |
| Keyword | Ascorbic acid, High Performance Liquid Chromatography, Brewery Spent Grain, Aspergillus flavus, Aspergillus tamarii |
| URL Website | www.tci-thaijo.org/index.php/fabjournal |
| Website title | www.tci-thaijo.org/index.php/fabjournal/article/view/110355 |
| ISSN | 2286-8615 |
| Abstract | This study evaluated the use of Brewery Spent Grain (BSG) as a novel substrate for the production of ascorbic acidusing Aspergillus flavusand Aspergillus tamarii.Spores of Aspergillus flavus andAspergillustamariiwere cultured in a liquid fermentation medium containing BSG (0.6% w/v) for ascorbic acid production. The process was studied at pH 48, temperature range 3045?C, agitation speed range 60160 rpm for 168 h. Stability studies and the effects of Ethylenediaminetetraacetic Acid (0.54.0 g/ml) on ascorbic acid productionwere studied. Ascorbic acid produced was quantified by titration techniques and with High Performance Liquid Chromatography (HPLC). The statistical analysis of the effects of temperature and agitation speed on ascorbic acid production showed no significant differenceat p<0.05. However, there was significant difference in the effect of pH on ascorbic acid production at p<0.05. Optimum ascorbic acid yieldsof7.25 g/L and 6.25 g/L wereproducedby A. tamariiand A. flavusrespectivelyat pH 5.0,temperature (40?C)and agitation speed of 100 rpm at 96 h of fermentation. High Performance Liquid Chromatography (HPLC) showed that6.248 g/L and 7.246 g/L of ascorbic acid wereproduced by A. flavusand A. tamarii,respectively.Ascorbic acid production by A. flavusand A. tamariiwas completely inhibited at 4 g/ml of EDTA.This study shows the potential of BSG as a novel substrate for ascorbic acid production. |
