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In vitroanti-tyrosinase activity of protein hydrolysate from spotted babylon(Babylonia areolata) |
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| รหัสดีโอไอ | |
| Creator | Phanuwat Prakot, Ninnaj Chaitanawisuti and Aphichart Karnchanatat |
| Title | In vitroanti-tyrosinase activity of protein hydrolysate from spotted babylon(Babylonia areolata) |
| Publisher | Faculty of Agro-Industry |
| Publication Year | 2558 |
| Journal Title | Food and Applied Bioscience |
| Journal Vol. | 3 |
| Journal No. | 2 |
| Page no. | 109-120 |
| Keyword | tyrosinase inhibitor, protein hydrolysate, spotted babylon |
| URL Website | www.tci-thaijo.org/index.php/fabjournal |
| Website title | www.tci-thaijo.org/index.php/fabjournal/article/view/78181 |
| ISSN | 2286-8615 |
| Abstract | Melanin is secreted by melanocyte cells distributed in the basal layer of the dermis. Melanin plays animportant role in protecting human skin from the harmful effects of ultra violet radiation. Tyrosinase is the key enzyme in melanin biosynthesis. In caseof over-activity can lead to dermatological disorder. The aim of this study was to investigate new tyrosinase inhibitorsof protein hydrolysate from spotted babylon. Protein hydrolysate was fractionated by ultrafiltration, MW < 5,000 Da showed the potencyas tyrosinase inhibitor with IC50value of 0.075?0.004 ?g/ml using L-DOPA as substrate which are strong about four times than the positive control kojic acid (IC50 = 0.251?0.007 ?g/ml). The tyrosinase inhibitory activity effect from retentate of5 kDa membrane of spotted babylon protein hydrolysate was moderately thermostable (showed maximum tyrosinase inhibitory activity about 90?C with 68% for 30 min), showed ?50% activity across a broad pH range of 312. In order to know the compounds responsible for tyrosinase inhibitory activities, the identification and characterization of protein hydrolysates of spotted babylon are currently underway in our laboratory. The search for more potent tyrosinase inhibitorsfrom protein hydrolysate are still neededfor the treatment of various dermatological disorders and are also applied as cosmetic whitening agents. |
