The effect of cooking process on antioxidant activities and total phenolic compounds of five colored beans
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Creator Priyanuch Chutipanyaporn, Kalyarat Kruawan, Chaowanee Chupeerach, Chalat Santivarangkna and Uthaiwan Suttisansanee
Title The effect of cooking process on antioxidant activities and total phenolic compounds of five colored beans
Publisher Faculty of Agro-Industry
Publication Year 2557
Journal Title Food and Applied Bioscience
Journal Vol. 2
Journal No. 3
Page no. 183-191
Keyword beans, antioxidant activity, 1-diphenyl-2-picrylhydrazyl assay, total phenolic compound, cooking process
URL Website www.tci-thaijo.org/index.php/fabjournal
Website title www.tci-thaijo.org/index.php/fabjournal/article/view/78051
ISSN 2286-8615
Abstract Beans are sources of many nutrients including proteins, carbohydrate, minerals, vitamins, dietary fiber and other bioactive compounds with protective and therapeutic effects towards oxidative stress and cell damage related diseases. Although many previous researches have been focused on antioxidant properties of beans, limited information on the comparison of five colored beans under cooking process regarding their antioxidant properties and total phenolic compounds (TPC) is currently available. Thus, the aim of this study was to investigate the effect of cooking process on antioxidant activities and TPC of five colored beans including mungbean, black bean, red kidney bean, white bean and soybean. Raw and beans cooked in boiling water for 20 min were freeze-dried and ground using a grinder. All samples were extracted with 70% (v/v) aqueous ethanol for 2 hours at 30 ?C. The antioxidant activities were analyzed using 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, and TPC was determined using FolinCiocalteu reagent. As results, the antioxidant activities and TPC of all five colored beans were elevated after the cooking process (DPPH values of 200-800 ?mole TE/100 g dry weight and 400-1500 ?mole TE/100 g dry weight of raw and cooked samples, respectively, and TPC values of 40-300 mg GAE/100 g dry weight and 80-300 mg GAE/100 g dry weight of raw and cooked samples, respectively). Black bean exhibited the highest TPC and DPPH scavenging activities, followed by red kidney bean, mung bean, soy bean and white bean, respectively. The high antioxidant activities and TPC in black bean might be due to the content of extracted bioactive compounds such as flavonol glycosides, anthocyanins and tannins. In addition, heat treatment greatly affected antioxidant activity and TPC of beans. This is possibly because of the disruption of plant cell walls during cooking, and sequentially, more bioactive compounds are released from the plant cells.
Chiang Mai University

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