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The comparison of villous damage at different ages of piglets infected with porcine epidemic diarrhea virus |
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| รหัสดีโอไอ | |
| Creator | 1. Prapassorn Boonsoongnern 2. Alongkot Boonsoongnern 3. Urai Pongchairerk 4. Tanatpon Paompa |
| Title | The comparison of villous damage at different ages of piglets infected with porcine epidemic diarrhea virus |
| Publisher | คณะสัตวแพทยศาสตร์ มหาวิทยาลัยเชียงใหม่ |
| Publication Year | 2561 |
| Journal Title | เชียงใหม่สัตวแพทยสาร |
| Journal Vol. | 16 |
| Journal No. | 1 |
| Page no. | 37-46 |
| Keyword | villous damage, piglets, porcine epidemic diarrhea virus |
| URL Website | http://www.vet.cmu.ac.th/cmvj/ |
| Website title | เชียงใหม่สัตวแพทยสาร |
| ISSN | 1685-9502 (print) 2465-4605 (online) |
| Abstract | An outbreak of porcine epidemic diarrhea (PED) is spreading in Thailand since 2007, leading to theloss of economy to pig farmers. The causative agent of this disease is porcine epidemic diarrhea virus(PEDV). The objective of this study was to investigate gross lesions and histopathology of the small intestineof PEDV-infected piglets of different ages. The three parts of the small intestine: duodenum, jejunum, andileum, were obtained from 3-day- and 10-day-old piglets infected with PEDV and normal piglets (control) forhistopathological study and immunohistochemical staining using antibodies against PEDV. The diagnosis ofPEDV infection was confirmed using Antigen Rapid TGE/PED Ag Test kit and reverse transcriptionpolymerase chain reaction (RT-PCR). All PEDV-infected piglets showed positive results, while the normalpiglets showed negative results. The immunohistochemistry indicated the presence of viral antigens in thecytoplasm of epithelial cells on the villi of the duodenum, jejunum, and ileum of piglets infected with PEDV ofall ages. Moreover, the ratio of villous height (VH) to crypt depth (CD) was observed to decrease in all parts ofthe small intestine, particularly jejunum of 3-day-old PEDV-infected piglets, compared to that of normal pigletsat the same age. A decreased ratio of VH:CD was observed in jejunum and ileum of 10-day-old pigletsinfected with PEDV. These findings indicated that younger piglets infected with PEDV showed more severeclinical signs, lesions, and villous damage than the older PEDV-infected piglets. |
