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Association analysis of candidate gene polymorphisms with egg production in Japanese quails (Coturnix japonica) |
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| รหัสดีโอไอ | |
| Creator | 1. Ly Thi Thu Lan 2. Nguyen Thi Hong Nhan 3. Dinh Thi Be Ngoc 4. Tran Trung Tin 5. Luu Huynh Anh 6. Nguyen Hong Xuan 7. Nguyen Trong Ngu |
| Title | Association analysis of candidate gene polymorphisms with egg production in Japanese quails (Coturnix japonica) |
| Publisher | คณะสัตวแพทยศาสตร์ มหาวิทยาลัยเชียงใหม่ |
| Publication Year | 2560 |
| Journal Title | เชียงใหม่สัตวแพทยสาร |
| Journal Vol. | 15 |
| Journal No. | 2 |
| Page no. | 117-125 |
| Keyword | Egg production, Japanese quail, genotype, mutation |
| ISSN | 1685-9502 (print) 2465-4605 (online) |
| Abstract | This study was conducted to identify genotypes and evaluate the effect of polymorphisms in growth hormone (GH), prolactin (PRL), bone morphogenetic protein receptor-Type IB (BMPR-1B) and melatoninreceptor-type 1C (MTNR-1C) genes on egg production of Japanese quail during 20 weeks of egg laying. PCR-RFLP and PCR-SSCP methods were used to determine the genotype of all genes. Our results showedthat the genotypes at loci C2161G in PRL and G294A in MTNR-1C were monomorphic as C and A, respectively. For the A237B in GH and A290T BMPR-1B mutations, two alleles were detected, giving three genotypes at each locus. In addition, the 24-bp difference of two genotype patterns provided an Indel-358mutation in PRL while three alleles were identified at locus A459TC (MTNR-1C). Taken together, the polymorphic sites did not affect egg production except the A459TC locus in MTNR-1C gene (P=0.002), wherequails carrying AA and CC genotypes provided the highest egg number (115.6-116.6 eggs/quail). Thus, the A459TC single nucleotide polymorphism is suggested to apply in other populations and it should beconsidered as a candidate marker for egg production. |
