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Enzymatic determination of the degree of chitosan deacetylation by family 18 chitinase |
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| รหัสดีโอไอ | |
| Creator | Sanya Kudan |
| Title | Enzymatic determination of the degree of chitosan deacetylation by family 18 chitinase |
| Contributor | Noppong Pongchaisirikul, Narongrit Dantragoon, Rath Pichyangkura |
| Publisher | Asia-Pacific Journal of Science and Technology |
| Publication Year | 2566 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 28 |
| Journal No. | 1 |
| Page no. | 6 |
| Keyword | Chitosan, Chitinase, DD, Hydrolysis, N-acetyl-D-glucosamine |
| URL Website | https://www.tci-thaijo.org/index.php/APST |
| Website title | https://so01.tci-thaijo.org/index.php/APST/article/view/264443 |
| ISSN | 2539-6293 |
| Abstract | The degree of chitosan deacetylation is the ratio of D-glucosamine (GlcN) units to the sum of N-acetyl-D-glucosamine (GlcNAc) and GlcN. An enzymatic method was developed to determine the percent degree of chitosan deacetylation (%DD) samples. A completely hydrolyzed chitosan solution was obtained by using crude chitinase from Bacillus licheniformis SK-1. The optimum condition was as follows: 20 mU of crude chitinase per milligram of chitosan in 0.1 M acetate buffer (pH 4.5) at 40 ?C for 8 h. The %DD was determined by generating a standard calibration curve. The total reducing sugar (TRS) and the %DD of standard chitosan samples (ranging from 43% to 100%) were determined by using nuclear magnetic resonance and colloidal titration methods. The standard calibration curve for determining the %DD was obtained with a linear correlation: %DD = ? 21.479 (TRS) + 102.45, r2 = 0.9933. The enzymatic method showed reproducible results and did not require special instruments. |
