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Antioxidant, toxic and cytotoxic activities of Butea superba Roxb |
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| รหัสดีโอไอ | |
| Creator | Jinnawat Manasathien |
| Title | Antioxidant, toxic and cytotoxic activities of Butea superba Roxb |
| Contributor | Piyanut Khanema |
| Publisher | Asia-Pacific Journal of Science and Technology |
| Publication Year | 2564 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 26 |
| Journal No. | 4 |
| Page no. | 12-Jan |
| Keyword | Butea superba, Antioxidant activity, Toxicity, Cytotoxicity, Apoptosis |
| URL Website | https://www.tci-thaijo.org/index.php/APST |
| Website title | https://so01.tci-thaijo.org/index.php/APST/article/view/255099 |
| ISSN | 2539-6293 |
| Abstract | This study investigated total phenolics content (TPC), total flavonoids content (TFC), antioxidant, toxicity, and cytotoxicity of B. superba ethanolic extract (BSE), which the plant is used in Thai traditional medicine. The toxicity was evaluated using a brine shrimp lethality assay and the cytotoxicity was done through Michigan Cancer Foundation (MCF-7) and prostate cancer (PC-3) cancer cell lines, particularly nuclear morphological changes and Deoxyribonucleic acid (DNA) fragmentation. The result found that BSE had low TPC and TFC (87.16 ตg gallic acid equivalent (GAE)/mg and 38.29 ตg CE/mg). The effective concentrations at 50% (EC50) and 99% (EC99) through 2,2-diphenyl-l-picrylhydrazyl (DPPH) and Ferric thiocyanate (FTC) assays were 3,610.10 (EC50/DPPH), 215.11 (EC50/FTC), 7,304.05 (EC99/DPPH), and 401.88 (EC99/FTC) ตg/mL, respectively. Lethal concentration at 1% (LC1) and 50% (LC50) were 54.63 and 184.24 ตg/mL Therapeutic index (TI) and margin of safety (MOS) assessment of BSE by DPPH and FTC assay were 0.051 (TIDPPH), 0.856 (TIFTC), 0.007 (MOSDPPH), and 0.136 (MOSFTC). BSE demonstrated an antiproliferative effects on MCF-7 and PC-3 cells in a dose-dependent manner, and induced apoptosis and DNA fragmentation in both cancer cells. In conclusion, BSE proposed to develop anticancer agents that could contribute to medicinal benefits. |
