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FLT3-ITD Mutations in Acute Myeloid Leukemia Patients in Northeast Thailand |
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| รหัสดีโอไอ | |
| Creator | 1. Piyawan Kumsaen 2. Goonnapa Fucharoen; Chittima Sirijerachai; Su-on Chainansamit; Nittaya Wisanuyothin; Pichayanan Kuwatjanakul; Surapon Wiangnon |
| Title | FLT3-ITD Mutations in Acute Myeloid Leukemia Patients in Northeast Thailand |
| Publisher | APJCP |
| Publication Year | 2559 |
| Journal Title | Asian Pacific Journal of Cancer Prevention |
| Journal Vol. | 17 |
| Journal No. | 9 |
| Page no. | 4395-4399 |
| Keyword | FLT3-ITD; acute myeloid leukemia; polymerase chain reaction |
| Abstract | The FLT3-ITD mutation is one of the most frequent genetic abnormalities in acute myeloid leukemia (AML) where it is associated with a poor prognosis. The FLT3-ITD mutation could, therefore, be a potential molecular prognostic marker important for risk-strati ed treatment options. We ampli ed the FLT3 gene at exon 14 and 15 in 52 AML patients (aged between 2 months and 74 years) from 4 referral centers (a university hospital and 3 regional hospitals in Northeast Thailand), using a simple PCR method. FLT3-ITD mutations were found in 10 patients (19.2%), being more common in adults than in children (21.1% vs. 14.3%) and more prevalent in patients with acute promyelocytic leukemia (AML-M3) than AML-non M3 (4 of 10 AML-M3 vs. 6 of 42 AML- non M3 patients). Duplication sequences varied in size-between 27 and 171 nucleotides (median=63.5) and in their location. FLT3-ITD mutations with common duplication sequences accounted for a signi cant percentage in AML patients in northeastern Thailand. This simple PCR method is feasible for routine laboratory practice and these data could help tailor use of the national protocol for AML. |
