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Preliminary Investigation on Arbutin Production inMulberry (Morus nigra) Callus Culture |
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| รหัสดีโอไอ | |
| Title | Preliminary Investigation on Arbutin Production inMulberry (Morus nigra) Callus Culture |
| Creator | Lalida Shank |
| Contributor | Piyathida Khaolueang, Srisulak Dheeranupattana, ChanidaphaThiraphatchotiphum, Thunwadee Limtharakul |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | Arbutin, Callus, Mulberry, Morus nigra |
| Abstract | 1. Arbutin 2. a phenolic glucoside 3. can be extracted from plants to use asbioactive compounds. This work investigated the production of arbutin incallus of mulberry 4. Morus nigra. Tissue culture of mulberry was carried outfrom the lateral buds via sterilization using different combinations ofchemicals. Soaking the explants in carbendazim for 10 min 5. followed by 70%ethanol (v/v) for 5 min, sodium hypochlorite (NaOCl) solution twice for 10min each and finally 0.1% (w/v) of mercuric chloride (HgCl2) for 5 minoffered the highest survival percentage of 72.50?17.08. The sterilized budswere subsequently placed in Murashige and Skoog media (MS) supplementedwith 2 6. 4-dichlorophenoxy (2 7. 4-D) at 1.0 mg/l for 4 weeks to establish theculture. The leaves from the sterile explants were directly transferred to MSsupplemented with 3 different growth regulators including 2 8. 4-dichlorophenoxy acetic (2 9. 4-D) 10. 1-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) at concentrations of 1.0 11. 2.0 and 3.0 mg/l to investigatearbutin production. Arbutin in callus culture was analyzed in comparison withnative leaves via high performance liquid chromatography (HPLC) on C18 HypersilODS column using water: methanal: hydrochloric acid (89:10:1 12. v/v) as mobilephase. The average arbutin content in callus cultured in MS supplementedwith 3.0 mg/l IAA was the highest at 35.67 ? 0.27 ?g/mg dry weight whencompared with the controls at 13.43 ? 4.29 ?g/mg dry weight and native leavesat 31.14 ? 13.86 ?g/mg dry weight. Due to the fewer pigments present in thecallus than in the native counterpart 13. the IAA elicited mulberry callus is 14. thus 15. an alternative source for arbutin production with the advantage of theseparation convenience. |
| Language | English |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
