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Purification and characterization of Pleurotus sajor-caju (Fr.) Sing gluco-oligosaccharide (Ps-GOS) obtained from Hevea ?1,3-glucanases digestion of insoluble ?-Glucans |
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| Title | Purification and characterization of Pleurotus sajor-caju (Fr.) Sing gluco-oligosaccharide (Ps-GOS) obtained from Hevea ?1,3-glucanases digestion of insoluble ?-Glucans |
| Creator | Teemaporn Anusarn |
| Contributor | Pannawich Sukprasirt, Rapepun Wititsuwannakul, Thanawat Pitakpornpreecha |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | Anti-Inflammation, ?-Glucan, Hevea Glucanase, RAW264.7 Macrophage |
| Abstract | The objective of this study was to purify and characterize of Pleurotus sajor-caju (Fr.) Sing gluco-oligosaccharide (Ps-GOS) obtained from the digestion of its insoluble ?- glucan with ?- 1, 3- glucanase derived from concentrated Hevea latex serum. A short-chains and water soluble Ps-GOS was characterized, with LC-MS and IR, and confirmed to be a mixture of ?-1, 3/1, 6-glucan. Further purification of Ps-GOS with anion -exchange (DEAEcellulose) and size-exclusion (Bio-Gel P10) chromatography were applied and obtain a various size of purified gluco-oligosaccharide with a molecular weight range from 1.5 to 20 kDa. In addition, our preliminary study was demonstrated that Ps-GOS could inhibit tumor growth and metastasis of human tumor, CaSki and B16F1 cell, xenografts in immunodeficient mice. Moreover, the immunohistochemistry study indicated that the level of inflammatory activity after mice were treated with Ps-GOS was decreased. Nevertheless, to further understand the anti-inflammatory mechanism, we will investigate its anti- inflammatory activities of purified Ps- GOS in lipopolysaccharide-induced RAW264.7 macrophage. |
| Language | EN |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
