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Identification and Characterization of Defensin-related Genes Challenged with Fungal Pathogen Infection in Cassava |
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| รหัสดีโอไอ | |
| Title | Identification and Characterization of Defensin-related Genes Challenged with Fungal Pathogen Infection in Cassava |
| Creator | Jarunya Narangajavana |
| Contributor | Pattaraporn Hormhuan, Budsakorn Dechapitak, Punchapat Sojikul, Unchera Viboonjun |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | Antimicrobial Peptides, Cassava, Cassava Anthracnose Disease, Defensin, Phytohormones |
| Abstract | Plant defensin family is one of the cysteine-rich antimicrobial peptides, which play important roles in plant immunity for responding against the invasion of pathogens. They are small cationic peptides which are shown to inhibit growth of a broad range of microorganisms. One of the major disease problems on production of cassava (Manihot esculenta Crantz) is cassava anthracnose disease, causing up to 80% yield loss by fungus Collectotrichum gloeosporiodes f. sp. manihotis (CAD). This research aims to identify and characterize defensin-related genes in cassava genome and investigated expression pattern of defensin genes upon CAD pathogen infection and to study the effect of phytohormone pre-treatment prior to fungal infection. The results of bioinformatics analysis showed that the coding sequences of 5 defensin gene members were identified in cassava genome and phylogenetic tree was constructed to verify each gene member. Also, defense-related response motifs were predicted in their promoter regions. Differential expression profiles of MeDef and MeDef1 . 2 were observed in CAD infection and phytohormone pre-treatment of the highly susceptible (HN) and less susceptible/ more resistant (HB60) cassava cultivars. The strong up-regulation of MeDef was found in HB60, whereas late induction of this gene was observed in HN. MeDef1 . 2 was not expressed. Salicylic acid pre-treatment prior to CAD infection could enhance defensive in HB60. This finding suggested that defensin has a potential function in CAD defense response in cassava, and further study should be useful for cassava improvement program to fungal pathogen resistance. |
| Language | EN |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
