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Anti-Breast Cancer Activity of Thai Herbal Medicine Formulation |
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| รหัสดีโอไอ | |
| Title | Anti-Breast Cancer Activity of Thai Herbal Medicine Formulation |
| Creator | Padchanee Sangthong |
| Contributor | Sadanon Siriphong, Puttinan Meepowpan |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | Anti-Cancer Activity, Cytotoxicity, Formulation, MCF-7 cells, Thai Traditional Medicine |
| Abstract | 1. Thai traditional medicine formulations have been used as folk medicine to treat breast cancer patients, balance element in the patient's body or increase their immunities. It is composed of five Thai medicinal plants 2. Albizia procera bark, Amphineurion marginatum root and stem, Diospyros mollis heartwood, Smilax corbularia and Smilax glabra rhizome. Nonetheless, biological testing for anti-cancer activity against breast cancer and biological effect of this formulation on cell line models have not yet been fully investigated. This research aimed to identify species of plant materials and investigate cytotoxic effects of the formulation and its constituent plants on normal mouse fibroblast (L929) and human breast cancer (MCF-7) cells. Phylogenetic identification of plant materials was evaluated using genetic markers on chloroplast DNA. Two non-coding regions 3. trnL intron and trnLF IGS, were amplified by PCR technique using universal primers. These PCR products were identified by DNA sequencing and aligned with ClustalW using BioEdit program. The nucleotide sequences were submitted to GenBank database and accession numbers were assigned. BLAST results of A. procera and A. marginatum showed the highest percent identities at 100% in both noncoding regions when compared with reference sequences on GenBank database. Then, ethanol maceration was performed which was similar to those practiced by Thai folk preparation. As the results, ethanolic extract of formulation had potent anti- cancer activity on MCF- 7 cells and low cytotoxicity on L929 cells with IC50 values of 52.51 and 81.48 ?g/mL, respectively. These results will support the biological evidences of Thai folk medicines to treat breast cancer cells. |
| Language | EN |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
