|
Effect of Metformin on Reducing Self-renewal and Proliferative Ability in Neuroblastoma SH-SY5Y Cells by Induction of Cell Differentiation |
|---|---|
| รหัสดีโอไอ | |
| Title | Effect of Metformin on Reducing Self-renewal and Proliferative Ability in Neuroblastoma SH-SY5Y Cells by Induction of Cell Differentiation |
| Creator | Pilaiwanwadee Hutamekalin |
| Contributor | Thunwa Binlateh, Supita Tanasawet |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | Neuroblastoma, Metformin, Self-Renewal, Differentiation, Proliferation |
| Abstract | Neuroblastoma (NB), the most common extracranial solid tumor, is challenged by high rate of relapse and drug-resistance. These may due to a presence of cancer stem cell (CSC) subpopulation in NB which relates to high capacity of self-renewal and differentiation. Recently, it has been believed that the promotion of cancer cells differentiation can irreversible change in the cancer phenotypes, particularly in loss of self-renewal. Consequently, drugs that trigger differentiation in cancers have currently being explored. In the present study, we showed the role of metformin in promoting differentiation and its effect on self-renewal capacity. The results revealed that metformin can promote cell differentiation with increasing neurite length and neuronal markers, ?-tubulin III and MAP-2. Furthermore, the ability of cell proliferation at day 2 and 3 after metformin-mediated cell differentiation was significantly diminished. Likewise, self- renewal capacity was decreased in metforminpromoted cell differentiation as determined by significant reduction of spheroid number and size. The percentage of cell in G0/ G1 phase was markedly increased in metformin-treated cells compared to control untreated cells. Together, Metformin has a potential role to promote differentiation and reduces self-renewal capacity and ability of cell proliferation after promoting differentiation. |
| Language | EN |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
