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Actinomycetes from Rice Field Soil, Nakonsawan and Their Antimicrobial Activity |
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| รหัสดีโอไอ | |
| Title | Actinomycetes from Rice Field Soil, Nakonsawan and Their Antimicrobial Activity |
| Creator | Thitiya Rattanakavil |
| Contributor | Khanungkan Klanbut |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | Actinomycetes, Rice Field Soil, 16s rRNA gene, Antimicrobial Activity |
| Abstract | A total of 100 actinomycetes isolates were isolated from rice field soil in Nong Bua, Nakonsawan, Thailand. All these strains were tested for preliminary and secondary screening for antimicrobial activity with test microorganisms including Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Bacillus subtilis ATCC 6633, Kocuria rhizophila ATCC 9341, Staphylococcus aureus ATCC 25923, Candida albicans ATCC 10231 and Xanthomonas oryzae X3. Partially purified extracellular and intracellular crude extracts were extracted using ethyl acetate and methanol as extraction solvents, respectively. Zones of inhibitions were obtained using disc diffusion method that contained 20 ?l of partially purified extracellular and intracellular extraction samples (50 mg/ml and 1 mg/ml) . The results showed that isolate NK13102 gave the highest inhibition zone in diameter against B . subtilis (26 mm), S . aureus (27 mm) K . rhizophila (29 mm) and X . oryzae (9 mm) . Analysis of 16S rRNA gene sequence and blast analyses confirmed that the isolates belonging to the genus Streptomyces with similarity to Streptomyces echinatus ( NK01105) , Streptomyces avellaneus (NK13102), Streptomyces bungoensis (NK14101) and Streptomyces laurentii (NK17205). |
| Language | EN |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
