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A Combination of 8 Bromo CyclicGMP with Neural Inducing Agents Could Efficiently Convert Human Adipose Stem Cells to Neural Progenitors |
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| รหัสดีโอไอ | |
| Title | A Combination of 8 Bromo CyclicGMP with Neural Inducing Agents Could Efficiently Convert Human Adipose Stem Cells to Neural Progenitors |
| Creator | Rangsun Parnpai |
| Contributor | Athip Limcharoen, Darawan Ruamkuson, Ton Yoisungnern, Mariena Ketudat-Cairns |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | Adipose Stem Cells, Mesenchymal, Neural Differentiation, NO-cGMP, Stem Cells, 8 Bromo cyclicGMP |
| Abstract | Human adipose stem cells (hASCs) among other stem cells offer a glimpse of possibilities in neurodegenerative disease treatment. By that fact, researchers have been developing neural transdifferentiation methods of hASCs over the last decades. Various types of inducing agents were applied to improve the protocol. NO-cGMP signaling pathway is known to play a vital role in regulation of cell growth, survival, differentiation, proliferation, migration, axon guidance and many other processes. cGMP, as the mediator, helps support NO regulation of cell survival, differentiation and neuroprotective regulation. Previous research demonstrated that applying NO donor agents in neural differentiation medium could convert hESCs into neural- like cells. However, neural differentiation through this specific signaling pathway on hASCs has not yet been investigated. In this study, 8 Bromo-cyclic GMP (8Br-cGMP) was added to neural induction cocktails to improve the neural transdifferentiation efficiency of hASCs. Results demonstrated that hASCs under this condition exhibited higher expression of neural genes and could further differentiate into mature neuronal and glial cells. Our findings highlighted that differentiated hASCs displayed neuronal progenitor profile, yet remained their plasticity as they can differentiate toward glial cells. Future studies employing in vivo transplantation models and their underlying mechanisms are warranted. |
| Language | EN |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
