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The pretreatment condition for chromosome count and karyotype analysis of Dimocarpus longan from Thailand |
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| รหัสดีโอไอ | |
| Title | The pretreatment condition for chromosome count and karyotype analysis of Dimocarpus longan from Thailand |
| Creator | Suparat Kunkeaw Lithanatudom |
| Contributor | Panurat Pipatchananan, Pathrapol Lithanatudom, Isara Patawang |
| Publisher | Mahasarakham University |
| Publication Year | 2564 |
| Keyword | chromosome preparation, chromosome number, karyotype, Dimocarpus longan |
| Abstract | Chromosome number and karyotype analysis reveal an important information for plant evolutionary study and plant breeding program. However, very few studies were conducted on cytogenetics of Dimocarpus longan (Longan), an economic important subtropical fruit in Thailand. This research aims to develop the practical procedure for longan chromosome preparation to investigate the chromosome number and karyotype analysis in 8 longan cultivars from Thailand. The chromosome preparation was optimized using longan shoot tip and root tip cells. Two pretreatment chemicals which are p-dichlorobenzene and 8-hydroxyquinoline were selected and treated at 4?C in different time points consisted of 1, 3, 6 and 24 hours. All treated samples were then hydrolysed in 1N HCl at 60?C for 7 minutes and stained with carbol fuchsin at room temperature for 15 minutes. The result showed that root sample pretreated with p-dichlorobenzene at 4?C for 1 hour was the best condition for longan chromosome analysis. This condition was further used for chromosome investigation in 8 Thai's longan cultivars which are Baiyoke, Plueakkhao, Phetsakorn, Krob-Ka-Ti, Haewkrae, E-daw, BiewKhiew Chiangmai and Pingpong. The chromosome number of all studied cultivars was determined as 2n = 30. The variation of the total chromosome length from 0.499 to 1.293 ?m while the relative length (RL) and size of chromosomes group L, M and S were observed among 8 longan cultivars. From this study, the pretreatment method can be used to investigate chromosomes of all longan cultivars. The data obtained from this study will be important information for plant breeders to develop longan varieties in Thailand. However, further chromosome investigation using approaches such as Fluorescence in situ hybridization (FISH) would be more informative for study evolution of longan in the future. |
| Language | English |
| URL Website | https://techno2.msu.ac.th/icofab2021/ |
| Website title | The 8th International Conference on Food Agriculture & Biotechnology |
