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Influence of urea on butanol production from sugarcane juice by Clostridium beijerinckii TISTR 1461 |
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| รหัสดีโอไอ | |
| Title | Influence of urea on butanol production from sugarcane juice by Clostridium beijerinckii TISTR 1461 |
| Creator | Kitipong Wechgama |
| Contributor | Supaporn Chunchom, Natanong Yodsing, Wiphusinee Worrachottiyanon, Kanlayani Charoensopharat |
| Publisher | Mahasarakham University |
| Publication Year | 2561 |
| Keyword | Clostridium beijerinckii TISTR 1461, Butanol production, Sugarcane juice, Urea |
| Abstract | 1. Nitrogen is the key factor influencing for butanol production. To improve the butanol production efficiency, the urea concentration was investigated for butanol production from sugarcane juice by Clostridium beijerinckii TISTR 1461. The addition of nutrients from P2 medium (excluding glucose) resulted in a butanol (9.29 g l-1) and acetone-butanol-ethanol (ABE) (12.27 g l-1) concentrations higher than the fermentation without supplemented with those in the sugarcane juice (butanol 2. 3.31 and ABE 3. 5.43 g l-1). The result suggested that some nutrients in P2 medium might be necessary for butanol production. Due to the high cost of yeast extract, urea was used as the nitrogen source in subsequent experiments. The juice supplemented with P2 medium (excepted glucose and yeast extract) and varied urea concentrations ranged from 0.27-1.35 g l-1 were used as butanol production media. The results showed that the butanol and ABE concentrations were increased with increasing the urea concentration from 0.27-0.81 g l-1. The highest butanol (9.41 g l-1) and ABE (11.78 g l-1) concentrations were observed at 0.81 g l-1 of urea concentrations and showed 0.30 of butanol yield and 0.20 g l-1 h-1 of productivity. The results concluded that urea promotes butanol production efficiency and suitable for instead of yeast extract. |
| Language | English |
