Indole-3-Acetic Acid Production of Chlorpyrifos Tolerant Bacteria Isolated from Agricultural Soils
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Title Indole-3-Acetic Acid Production of Chlorpyrifos Tolerant Bacteria Isolated from Agricultural Soils
Creator Thanakorn Saengsanga
Contributor Nattaphon Naboon, Srisuda Kamwan, Tarntip Rattana
Publisher Mahasarakham University
Publication Year 2561
Keyword Chlorpyrifos, Organophosphate, Indole-3-acetic acid (IAA), IAA producing bacteria, Chlorpyrifos tolerant bacteria
Abstract Chlorpyrifos, an organophosphate insecticide, has been widely used to kill a number of pests including insects and worms. The research work was carried out to isolate chlorpyrifos tolerance bacteria from agricultural soil. Forty-seven of chlorpyrifos tolerant bacterial isolates were able to grown in the medium amended with 150 mg L-1 of chlorpyrifos. Investigation of indole-3-acetic acid (IAA) production of these isolates was performed. Nine isolates of chlorpyrifos tolerant bacteria, named BW1, BW3, BW7, BW9, BW11, TV11, TV24, BK27 and BK29 were capable to synthesize IAA. Out of them BW9 gave the highest amount of IAA (168.46 ?g mL-1) followed by BW3 (104.66 ?g mL-1) in culture media supplemented with L-tryptophan. Furthermore, IAA production of all strains was determined in a medium supplemented with 100 mg L-1 of chlorpyrifos and L-tryptophan. The result revealed that chlorpyrifos had no significant effect on IAA production of all isolates except isolate BW9. Our result highlight that these bacteria would be investigated further on the chlorpyrifos biodegradation and plant growth stimulation for application in environmental and agricultural aspects.
Language English
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