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Functional analysis of Cis Regulatory Elements (CREs) in pollen specific/preferential genes |
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| รหัสดีโอไอ | |
| Title | Functional analysis of Cis Regulatory Elements (CREs) in pollen specific/preferential genes |
| Creator | Tien Dung Nguyen Thi Mai Tran Xuan Vu Nguyen Bang Phuong Pham |
| Contributor | Faculty of Biotechnology and Food technology Thai Nguyen University of Agriculture and Forestry TUAF Thai Nguyen Vietnam. |
| Publisher | Mahasarakham University |
| Publication Year | 2560 |
| Keyword | CREs rice pollen, promoter |
| Abstract | Promoter is a region of DNA located in 5' upstream region from the transcription start site (TSS) of gene that has been known as play a critical role in regulating gene expression and widely used as an ideal tools for crop improvement and analysis of gene functions. Recently, based on microarray data we have identified 20 pollen specific or preferred promoters from rice (Oryza sativa L.), including 9 early microspore (RMP1 through 9) and 11 late pollen specific promoters (OsLPS1 through 11) (Nguyen et al. 2015, 2016a,b). Moreover, detailed analysis promoter sequencens revealing several cis-regulatory elements (CREs) involved in tissues, organs-specific or preferrential expression such as GTGANTG10, POLLEN1LeLAT52. However, not only CREs but also novel motifs or enhancer elements involved in specific expression also need to be concerned. In order to better understand the function of CREs, these promoters are being studied in detail using the method of deletion analysis in transgenic plants, rice and Arabidopsis. Our study will be useful for understading the roles of CREs in regulating specific expression of gene in pollen grains, and also provide new set of pollen specific promoters for plant breeders in Vietnam. |
